Abstract: ob＜x＞jective To detect the changes of gene ex＜x＞pression caused by LASP1 in hepatoma cells, which could provide more basis for further study of the molecular mechanisms associated with the development of hepatocellular carcinoma mediated by LASP1. Methods The eukaryotic ex＜x＞pression plasmids of LASP1 were constructed, and transfected into HepG2 cells, to establish the hepatoma cells overexpressing LASP1 and related control cells; the gene ex＜x＞pression profiles of the two groups of cells were detected by transc＜x＞riptome sequencing technology; the differentially expressed genes (DEGs) between the two groups of cells were compared by bioinformatics analysis. GO, KEGG pathway and WikiPathway enrichment analysis were used, to explore the biological process and related pathways related to the DEGs regulated by LASP1; ba＜x＞sed on the STRING databa＜x＞se, the interaction network of proteins encoded by the DEGs, and the potential key genes regulated by lasp1was also explored. Results Compared with the control cells, 410 DEGs were screened out in hepatoma cells overexpressing LASP1. 308 DEGs were up-regulated and 102 DEGs down-regulated. The results of GO enrichment analysis showed that the DEGs were related to the biological processes of ion transmembrane transport, cation transport, and et al. The results of KEGG pathway analysis showed that the DEGs were mainly associated with chemical carcinogenesis and et al. The results of WikiPathway analysis showed that the DEGs were related to histone modification and et al. The proteins encoded by DEGs could form a complex protein interaction network, and the genes, including HIST1H1B, HIST1H4B, NCAM1, SLC17A7 were potential key genes regulated by LASP1. Conclusions This study reveals the genes regulated by LASP1 in hepatoma cells and their involved biological processes and related pathways. The finding provides a basis for further study of the biological functions and related molecular mechanisms mediated by LASP1 in hepatoma cells.