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    刘明敏, 郑亚茹, 何玉凤, 索峰, 缑灵山, 李桂林. 子宫腺肌病病灶内膜中细胞黏附因子表达变化特征及TNF-α/NF-κB信号通路的调控作用[J]. 徐州医科大学学报, 2021, 41(11): 805-809. DOI: 10.3969/j.issn.2096-3882.2021.11.005
    引用本文: 刘明敏, 郑亚茹, 何玉凤, 索峰, 缑灵山, 李桂林. 子宫腺肌病病灶内膜中细胞黏附因子表达变化特征及TNF-α/NF-κB信号通路的调控作用[J]. 徐州医科大学学报, 2021, 41(11): 805-809. DOI: 10.3969/j.issn.2096-3882.2021.11.005
    shu
    ex＜x＞pression characteristics of cell adhesion factors in ectopic endometrium of patient with adenomyosis and the potential regulatory role of of TNF-α/NF-κB signaling[J]. Journal of Xuzhou Medical University, 2021, 41(11): 805-809. DOI: 10.3969/j.issn.2096-3882.2021.11.005
    Citation: ex＜x＞pression characteristics of cell adhesion factors in ectopic endometrium of patient with adenomyosis and the potential regulatory role of of TNF-α/NF-κB signaling[J]. Journal of Xuzhou Medical University, 2021, 41(11): 805-809. DOI: 10.3969/j.issn.2096-3882.2021.11.005
    shu

    子宫腺肌病病灶内膜中细胞黏附因子表达变化特征及TNF-α/NF-κB信号通路的调控作用

    ex＜x＞pression characteristics of cell adhesion factors in ectopic endometrium of patient with adenomyosis and the potential regulatory role of of TNF-α/NF-κB signaling

      摘要
    • 摘要: 目的探讨细胞黏附因子在子宫腺肌病病灶内膜中的表达及调控机制。方法选择2019年12月—2020年6月在徐州医科大学附属徐州妇幼保健院确诊为子宫腺肌病患者20例（病灶内膜组），另选择因宫颈癌前病变行全子宫切除患者19例作为对照组，采集相应组织病灶标本。比较病灶内膜与对照组子宫内膜中钙黏附蛋白、选择素及其他细胞黏附因子的表达水平，同时检测肿瘤坏死因子α (TNF-α)和核因子κB（NF-κB）P65蛋白表达变化。结果子宫腺肌病病灶内膜中钙黏附蛋白N (N-cadherin)、血管内皮钙黏附蛋白(VE-cadherin)、选择素L(L-selectin)、血管细胞黏附因1 (VCAM-1)、Ⅰ型跨膜糖蛋白(CD22) 和神经生长调节蛋白1 (NEGR1) mRNA与对照组相比显著升高(P<0.05)，而钙黏附蛋白E(E-cadherin) mRNA水平较对照组明显降低(P<0.05)，2组间选择素P和选择素E( E-selectin) mRNA表达水平差异无统计学意义(P＞0.05)。另外病灶内膜中TNF-α和NF-κB P65蛋白水平较对照组亦显著增加(P<0.05)。结论不同细胞黏附因子在子宫腺肌病病灶内膜中的表达改变及变化程度存在差异，TNF-α/NF-κB信号通路的活化可能与内膜细胞中细胞黏附因子异常表达有关。

       

      Abstract: ob＜x＞jective To investigate the abnormal ex＜x＞pression of cell adhesion factors in ectopic endometrium of adenomyosis. Methods This study was approved by Xuzhou Maternity and Child Health Care Hospital. A total of 20 patients with adenomyosis from December 2019 to June 2020 in our hospital were recruited into this study. Moreover, 19 patients who underwent hysterectomy due to cervical precancerous lesions. Quantitative real time PCR was used to determine the ex＜x＞pression of cadherin, selectin and other cell adhesion factors ex＜x＞pression in endometrium. Moreover, western blot was used to identify the ex＜x＞pression of TNF-α and NF-κB P65 protein. Results In comparison with control group, the ex＜x＞pression of N-cadherin, VE-cadherin, L-selectin, VCAM-1, CD22, and NEGR1 mRNA in ectopic endometrium of patients with adenomyosis were higher ( P< 0.05). However, the ex＜x＞pression of E-cadherin was lower than that of control group (P< 0.05). There were no significant differences for P-selectin and E-selectin mRNA ex＜x＞pression between these two groups. Moreover, the ex＜x＞pression of TNF-αand NF-κB P65 protein in ectopic endometrium of patients with adenomyosis was higher than that of control group. Conclusion There were differences for the alterations of cell adhesion factors between the ectopic endometrium of patients with adenomyosis and the eutopic endometrium of control group. The activation of TNF-α/NF-κB signaling pathway might be associated with the abnormal ex＜x＞pression of cell adhesion factors in ectopic endometrium of adenomyosis

       

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