Abstract: Purpose: To explore parameters that influence liver stiffness measurement (LSM) using visual transient elastography (ViTE) in healthy population. Methods: We use Mindray’s Resona6 ViTE technology to randomly test the liver stiffness of healthy adults, and 40 subjects was selected to analyze the impacts of the body position, probe’s position and direction on LSM. Besides, We assessed the impacts of age, gender, height, weight, BMI, and subcutaneous fat thickness on LSM. Results: The LSM values from the supine and the left decubitus position were (4.99±1.22) Kpa and (6.56±3.06) Kpa respectively, and the difference was statistically significant. The LSM values obtained from the right intercostal space and the right inferior margin of costal arch are (4.99±1.22) Kpa and (4.43±1.46) Kpa respectively, and the difference was statistically significant. The LSM values obtained when the probe is perpendicular to the chest wall, at an angle of 15° to the vertical direction of the chest wall, and at an angle of 30° to the vertical direction of the chest wall were (4.99±1.22) Kpa, (5.20±1.53) Kpa, (6.04±2.41) Kpa respectively, and the difference was also statistically significant. This study also found no significant impact of age, gender, height, weight, BMI, and subcutaneous fat thickness on LSM. Moreover, the detection success rate of the right lobe of liver is significantly higher than that of left lobe. Conclusion: When applying ViTE technology to detect liver stiffness, the influence of subject’s body position, probe’s direction and position on the LSM value should be considered.