Abstract: ob＜x＞jective: To investigate the effect of clinical pharmacist intervention on prophylactic use of antibiotics in type II incision in hepatopancreatobiliary surgery department. Methods: The medical records of type II incision surgeries in the same period in 2019 and 2020 were collected and divided into the pre-intervention group and the post-intervention group. The data of the two groups were statistically analyzed and evaluated according to the evaluation index of prophylactic use of antibiotics in perioperative period of type II incision surgery. The selection, timing and course of prophylactic use of antibiotics were compared between the two groups, and the top five varieties of antibiotics were observed in the two groups. Results: Species selection error rate from 84.20% to 23.92%, the prevention of drug varieties replacement error rate from 15.35% to 2.29%, no indications combination fell from 58.92% to 15.01%, drug use after the surgery decreased from 65.24% to 24.43% , preoperative > 2 h drug use decreased from 31.38% to 13.74%, medical treatment > 48 h decreased from 92.1% to 14.25%, and the differences were statistically significant (P < 0.05)。The dosage of compound preparations containing enzyme inhibitors and fluoroquinolones was decreased after intervention, and the utilization rate of the second generation cephalosporins was increased. Conclusion: The effective pharmaceutical intervention by clinical pharmacists can improve the rationality of prophylactic use of antibiotics in type II incision in hepatopancreatobiliary surgery department.