Abstract: O bjective The eukaryotic ex＜x＞pression plasmid of Apobec-1 complementary factor (A1CF) was constructed and transfected into A549 and H 1299 of lung cancer cells. Methods Total RNA from renal cancer cell 786-o was e xtracted. Then, human A1CF gene was amplified by RT-PCR and inserted into pcDNA3.1(+) vector by enzyme digestion method. The reconstructed plasmid was identified by BamHI and XbaI , DNA sequence sequencing, and then transfected into lung cancer cells. The ex＜x＞pression of A1CF was detected by Western blotting. The effect of A1CF on the migration ability of lung cancer was observed with scratch and Transwell laboratory experiments.Results The results of enzyme digestion and sequencing showed that the sequence of fragments inserted in pcDNA3.1(+)-A1CF was consistent with human A1CF sequence. Western blotting showed that the protein ex＜x＞pression of A1CF in A549 and H 1299 cells increased and the ex＜x＞pression levels of E-cadherin and P-ERK also increased significantly. Conclusion pcDNA3.1(+)-A1CF of eukaryotic ex＜x＞pression plasmid was constructed successfully .And A1CF promoting lung cancer cell migration may be associated with phosphorylation of ERK