Abstract: Objective: To evaluate the expression of miR-210-3p in lung cancer tissues and its effect on prognosis. Methods: 187 cases of lung cancer patients diagnosed and treated in our hospital during February 2012 to December 2013 were selected as subjects for clinical study, and their clinical data were retrospectively analyzed. The relative expression levels of miR-210-3p in lung cancer tissues and adjacent tissues of lung cancer patients were compared, and the relationship between the relative expression levels of miR-210-3p and various clinicopathological data of patients was analyzed. Meanwhile, the survival curves of patients were statistically analyzed. Results: The relative expression of miR-210-3p in lung cancer tissues was significantly higher than that in adjacent tissues (t=10.847,P=0.001).The expression level of miR-210-3p in lung cancer tissues was related to the clinical classification and lymph node metastasis of lung cancer (P<0.05), and was not related to the gender, age, smoking history, lung cancer tissue type and tumor tissue size of lung cancer patients (P>0.05).Clinical stage for Ⅲ/Ⅳ, with lymph node metastasis of lung cancer patients with miR-210-3p expression level was significantly higher clinical stage for Ⅰ/Ⅱ, without lymph node metastasis of lung cancer patients (P<0.05).The 1-year, 3-year and 5-year survival rates of patients with high miR-210-3p expression were significantly lower than those with low miR-210-3p expression (P<0.05).Conclusion: The relative expression level of miR-210-3p in lung cancer tissues was higher than that in adjacent tissues, and the expression level of miR-210-3p in lung cancer tissues was related to the clinical classification and lymph node metastasis of lung cancer, and had no relation to the gender, age, smoking history, lung cancer tissue type and tumor tissue size of lung cancer patients. The survival rate of patients with high mir-210-3p expression was lower than that of patients with low miR-210-3p expression.