Abstract:
ob<x>jective To understand the drug resistance and pathogenicity characteristics of non-O1/O139 Vibrio cholerae carrying the vcsN2 gene of the three-type secretion system. Methods The stools of patients with diarrhea suspected of Vibrio cholerae infection were cultured, and the VITEK 2 Compact automatic identification drug susceptibility instrument was used for biochemical identification. Finally, the 16s rDNA of the bacteria was extracted and sequenced for homology comparison. The KB method was used to detect the drug resistance of Vibrio cholerae, and the PCR method was used to detect the virulence-related genes of Vibrio cholerae. Results The isolated bacteria showed blue-green colony characteristics in the Vibrio chromogenic medium. The blood plate was a milky white colony with hemolysis, which was positive for oxidase. Obtuse round and curved gram-negative bacilli at both ends were seen under the microscope. The biochemical identification was Vibrio cholerae. The homology comparison was performed after 16s rDNA sequencing, and the similarity was 99.79%. The serum agglutination test showed that it was non-O1/O139 Vibrio cholerae. Drug sensitivity results showed that the strain was sensitive to most antibiotics, including compound trimethoprim, amikacin, levofloxacin, ciprofloxacin, ampicillin, cefepime, ceftriaxone, tetracycline, gentamicin, It is resistant to cefazolin, clindamycin and imipenem. The virulence test results showed that in addition to the vcsN2 gene of the three-type secretion system, it also carried rtxC, hlyA, toxR , and ompW. The detected O1/O139 rfb, tcpA, ctxA, and chx were all negative. Conclusion This clinically isolated strain of non-O1/O139 Vibrio cholerae carries many virulence genes. It is necessary to strengthen the detection of non-O1/O139 Vibrio cholerae virulence and drug resistance genes in patients with intestinal outpatient diarrhea. The prevention, control and treatment of bacteria provide a certain reference basis.