Abstract: ob＜x＞jective To investigate the effect of NDRG3 on the proliferation, migration and invasion ability of ovarian cancer cell line SKOV3 and its mechanism. Methods The ex＜x＞pression of NDRG3 in ovarian cancer and normal ovarian tissues was analyzed using TCGA databa＜x＞se. The GEPIA databa＜x＞se was used to analyze the effect of NDRG3 ex＜x＞pression on the overall survival of patients with ovarian cancer. The ex＜x＞pression of NDRG3 in ovarian cancer and normal ovarian tissues was detected by quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot. The ex＜x＞pression of NDRG3 gene in ovarian cancer cell SKOV3 was silenced by lentivirus-med iated transfection, then the level of NDRG3 protein ex＜x＞pression in ovarian cancer cells was detected by Western blot. The abilities of proliferation , migration and invasion of SKOV3 cells were detected by plate colony formation assay as well as CCK-8 , scratch assay and Transwell assay respectively. We use the technology of Western blot to detect the effect of NDRG3 on the ex＜x＞pression of MMP-2，MMP-9 and N-cadherin. Results The protein level of NDRG3 in human ovarian cancer tissue was higher than that in normal ovarian tissue. SKOV3 cells shows remarkable decrease in proliferation , migration and invasion abilities , and the level of MMP-2, MMP-9 and N-cadherin witnessed a significant decline after NDRG3 was down-regulated ( P<0.05). Conclusion NDRG3 is highly expressed in ovarian cancer tissue. The silence of NDRG3 can inhibit the abilities of proliferation , migration and invasion of human ovarian cancer cell SKOV3.