Abstract:
ob<x>jective To investigate the effect and possible mechanism of ADRM1 on proliferation and me<x>tastasis of pancreatic adenocarcinoma cells . Methods GEPIA databa<x>se was used to analyze the mRNA level of ADRM1 in pancreatic adenocarcinoma and its effect on clinical prognosis. Effects of inhibition or overex<x>pression of ADRM1 on proliferation and me<x>tastasis of pancreatic cancer cells were analyzed by EdU, CCK-8 and Transwell assays. Bioassay and Western blot were used to investigate the mechanism of ADRM1 regulating the proliferation and me<x>tastasis of pancreatic cancer cells. Results The mRNA level of ADRM1 was up-regulated in pancreatic cancer, which was not correlated with overall survival, but was negatively correlated with disease-free survival. Inhibition of ADRM1 c ould weaken the proliferation and me<x>tastasis of pancreatic cancer cells, while overex<x>pression of ADRM1 could enhance the proliferation and me<x>tastasis of pancreatic cancer cells. ADRM1 could activate the JAK tyrosine protein kinase 2-signal transduction and transc<x>ription activator 3 (JAK2-STAT3) signaling pathway. Conclusion As an oncogene, ADRM1 is up-regulated in pancreatic adenocarcinoma and promotes the proliferation and me<x>tastasis of cells, which may be related to the activation of JAK2-STAT3 signaling pathway.