19 665例人乳头瘤病毒感染类别、多重感染和递减顺序分析
Analysis of human papillomavirus infection status、genotype distribution、mutli-infection patterns and infection rate decrease in sequence among 19665 cases in Huaian region
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摘要: 目的 分析大样本机会性的淮安市妇女宫颈感染人乳头瘤病毒（Human papilIomavirus HPV)的现状、基因亚型、感染递减顺序、多重亚型 感染和递减顺序等。方法 收集 2010 年 1 月 1 日至 2021年 12月 30 日就诊于淮安市妇幼保健院妇科门诊检查HPV标本21992例，采用实时荧光 PCR 技术定性检测，剔除检查结果重复HPV标本后共19665份样本。研究分析HPV感染总检出率、各亚型分布、感染递减顺序、 多重亚型 感染及递减顺序特征等。结果 HPV 总检出率为 28. 70% ，其中高危型检出率是 21.15%，高危亚型感染率递减顺序HPV52 3. 88%、HPV16 3. 72%、HPV58 2. 47%、HPV53 1. 80%、HPV39 1. 34%、HPV51 1. 26%、HPV18 1. 21%、HPV33 1. 05%、HPV31 0. 84%、HPV56 0. 72%、HPV59 0. 69%、HPV68 0. 69%、HPV45 0. 55%、HPV66 0. 44%、HPV35 0. 18%、HPV73 0. 17%、HPV82 0. 14%。低危型 检出率是 7. 55%，低危亚型 感染率由高至低顺序HPV81 2. 14%、HPV42 1. 65%、HPV 43 1.36%、HP44 1. 29%、HPV6 0. 67%、HPV11 0. 42%、HPV53 0. 02%。混合型检出率是 4. 56%。结论 淮安地区妇女机会性高危HPV检测感染率为21.15% ,前6位高危型HPV感染是 52、16、58、53、39、51 ，后5位是45、66、35、73、82。高危HPV感染以单一亚型为主，两种亚型 也占一定比例。7种低危型HPV感染前4位81、42、43、44，HPV6、11分别感染率分别占第5和第6位。通过分析本地区患者 HPV 感染状况及 HPV 基因亚型分布特点等特征，对 预防区域宫颈病变的HPV 疫苗型别选择以及 HPV 感染精准防治有重要指导价值。Abstract: ob＜x＞jective ： To investigate characterictic and patterns of subtypes and multiple infenction of human papilIomavirus(HPV) of large cases in femaIe outpatients of gynecology clinic. Methods: Real-time polymerase chain reaction (RT-PCR)was used to detect total HPV infection rate 、 HPV gene subtype distribution 、high-risk HPV and low-risk HPV patterns 、muliti-infections and feature of decrease in sequence among 19665 femaIe outpatients of gynecology clinic in Huaian Maternal and Child Health-Care hospital from January 1 ，2010 to Decenber 30 ，2020 . Results: The total positive rate of HPV was28.70%. The infection rate of high-risk subtype was 21.15%. It is HPV52 3. 88%、HPV16 3. 72%、HPV58 2. 47%、HPV53 1. 80%、HPV39 1. 34%、HPV51 1. 26%、HPV18 1. 21%、HPV33 1. 05%、HPV31 0. 84%、HPV56 0. 72%、HPV59 0. 69%、HPV68 0. 69%、HPV45 0. 55%、HPV66 0. 44%、HPV35 0. 18%、HPV73 0. 17%、HPV82 0. 14% that the positive rate of high-risk subtype of HPV declined in sequence.The positive rate of low-risk subtype was 7. 55%. It is HPV81 2. 14%、HPV42 1. 65%、HPV43 1.36%、HP44 1. 29%、HPV6 0. 67%、HPV11 0. 42%、HPV53 0. 02% that the positive rate of low-risk subtype of HPV decrea sed in sequence. The positive rates of HPV combined high-risk subtype with low-risk subtype was 4. 56%. Conclusions: In Huaian region ，The infection rate of high-risk subtype was 21.15% in femaIe outpatients of gynecology clinic . The top six high-risk subtypes were HPV 52、16、58、53、39 and 51，whereas bottom five high-risk subtypes were HPV 45 、66、73、82 and 35. The single high-risk subtypeb HPV infection was predominant ，double high-risk subtypeb HPV infection accounted for less proportion. The top four low-risk subtypes were HPV 81 、42、43 and 44，however，infection rate of low-risk subtypes ranked HPV 6 a nd 11 in sixth and seventh position respectively. Comprehensively investigating human papillomavirus infection status 、genotype distribution and mutli-infection patterns guides us the vaccine development and application and leads scientific and precise prevention of HPV infection
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