Abstract: ob＜x＞jective To explore the value and the relevance of three-dimensional arterial spin-labeling imaging (3D ASL) and dynamic susceptibility contrast perfusion weighted Imaging (DSC-PWI) in the preoperative grading of brain glioma. Methods 39 pathologically confirmed brain glioma cases who underwent 3D ASL and DSC-PWI were retrospectively reviewed. 3D ASL cerebral blood flow (ASL-CBF), DSC cerebral blood flow (DSC-CBF), DSC cerebral blood volume (DSC-CBV) values and with the contralateral normal brain white matter after correction of the corresponding 3D ASL-relative cerebral blood flow (ASL-rCBF), DSC-relative cerebral blood flow (DSC-rCBF), DSC-relative cerebral blood volume (DSC-rCBV) parameter values were recorded. Results Our research found that ASL-CBF, DSC-CBF, DSC-CBV and relative values increased with higher tumor grade, There was significant positive correlation between ASL-CBF and DSC-CBF, as well as between ASL-rCBF and DSC-rCBF, And there was a moderate degree of positive correlation between ASL-CBF and DSC-CBV, as well as between ASL-rCBF and DSC-rCBV, ASL-CBF, DSC-CBF, DSC-CBV and their relative values had a significant positive correlation with pathological grade. The ROC curve showd that, DSC-rCBF had the largest AUC, followed by DSC-rCBV, ASL-rCBF. Conclusion Thus, both 3D ASL and DSC-PWI quantitative parameters have high sensitivity and specificity in evaluating blood perfusion in glioma, and both can be used for preoperative hemodynamic evaluation of glioma. 3D ASL is simple and easy to perform, and can effectively assess the preoperative grading of glioma.