Abstract: Background: This article aimed to increase the production of Mesenchymal stem cell exosomes, and enhance their biological activity to promote healing and regeneration.Methods: We cultured Mesenchymal stem cells with the Nocardia rubra cell wall skeleton and then extracted exosomes. After that, we identified exosomes ’ morphology and surface marker protein. In addition, we measured the protein of exosomes to compare yield changes, detected the proliferation and migration of Human Dermal Fibroblast by CCK-8 and scratch assay, and tested the ability of exosomes to promote Human Umbilical Vein Endothelial Cells regeneration by in vitro angiogenesis assay. Results: We found that cultivating Mesenchymal stem cells with Nocardia rubra cell wall skeleton significantly increased the production of Mesenchymal stem cell exosomes. It is reassuring enough that the red card did not alter the classical morphology and surface marker proteins of MSC exosomes. Moreover, the ability of exosomes to promote HDF proliferation and migration was strongly enhanced. Furthermore, the activity of exosomes in promoting vascular regeneration has also been greatly improved. Conclusions: In conclusion, the Nocardia rubra cell wall skeleton can improve the production of Mesenchymal stem cell exosomes and enhance their biological activity to promote healing and regeneration.