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    沈莉, 李玉静, 丁锦红, 李强. 膜蛋白CD81增强滋养细胞的黏附能力及机制研究[J]. 徐州医科大学学报, 2023, 43(12): 869-873. DOI: 10.3969/j.issn.2096-3882.2023.12.002
    引用本文: 沈莉, 李玉静, 丁锦红, 李强. 膜蛋白CD81增强滋养细胞的黏附能力及机制研究[J]. 徐州医科大学学报, 2023, 43(12): 869-873. DOI: 10.3969/j.issn.2096-3882.2023.12.002
    shu
    SHEN Li, LI Yujing, DING Jinhong, LI Qiang. CD81 enhances the adhesion of trophoblasts and related mechanisms[J]. Journal of Xuzhou Medical University, 2023, 43(12): 869-873. DOI: 10.3969/j.issn.2096-3882.2023.12.002
    Citation: SHEN Li, LI Yujing, DING Jinhong, LI Qiang. CD81 enhances the adhesion of trophoblasts and related mechanisms[J]. Journal of Xuzhou Medical University, 2023, 43(12): 869-873. DOI: 10.3969/j.issn.2096-3882.2023.12.002
    shu

    膜蛋白CD81增强滋养细胞的黏附能力及机制研究

    CD81 enhances the adhesion of trophoblasts and related mechanisms

      摘要
    • 摘要: 目的 探讨CD81对滋养细胞黏附能力的影响及可能机制，以便阐明CD81参与子痫前期发病的具体机制。方法 体外培养永生化的人孕早期绒毛外滋养细胞系HTR-8/SVneo细胞，并感染高表达CD81的腺病毒（Ad-CD81）或转染抑制内源性CD81的干扰RNA（si-CD81）。采用细胞黏附实验检测HTR-8/SVneo细胞的黏附能力，采用免疫印迹法检测HTR-8/SVneo细胞中黏着斑激酶（FAK）的表达情况。结果 与感染Ad-CTL的细胞相比，感染Ad-CD81的HTR-8/SVneo细胞黏附能力增强，570 nm处的光密度值显著增加，差异有统计学意义（P<0.05）。与转染si-CTL的细胞相比，转染si-CD81的HTR-8/SVneo细胞黏附能力减弱，570 nm处的光密度值显著下降，差异有统计学意义（P<0.05）。随着Ad-CD81浓度的增加，感染Ad-CD81的HTR-8/SVneo细胞中FAK的蛋白表达较Ad-CTL感染细胞逐渐下降（P<0.05或P<0.01）。随着si-CD81浓度的增加，转染si-CD81的HTR-8/SVneo细胞中FAK的蛋白表达较si-CTL转染细胞逐渐增加（P<0.05或P<0.01）。结论 膜蛋白CD81可增强滋养细胞的黏附能力，其分子机制可能与CD81抑制滋养细胞中FAK的蛋白表达有关。

       

      Abstract: Objective To explore the effect of CD81 on trophoblast adhesion and related mechanisms, in order to determine the role of CD81 in the pathogenesis of preeclampsia.Methods A human first-trimester-derived trophoblast cell line (HTR-8/SVneo cells) was cultivated in vitro before transfection with adenovirus Ad-CD81 which highly expressed CD81 or si-CD81 which inhibited endogenous CD81 through interference RNA. Then, the ability of cell adhesion was detected by adhesion assay. The expression of focal adhesion kinase (FAK) in HTR-8/SVneo cells was detected by Western blot. Results Compared with Ad-CTL-infected cells, Ad-CD81- infected HTR-8/SVneo cells showed enhanced adhesion ability and increased optical density at 570 nm (P<0.05). Compared with si-CTL-transfected cells, si-CTL-transfected HTR-8/SVneo cells presented weakened adhesion ability and decreased optical density at 570 nm (P<0.05). Furthermore, with the increase of concentration gradients, the expression of FAK was down-regulated in the Ad-CD81-infected HTR-8/SVneo cells, but up-regulated in the si-CD81-transfected cells(P<0.05 or P<0.01).Conclusions CD81 can enhance the adhesion of trophoblasts, which may be associated with its inhibitory effect on FAK expression in trophoblasts.

       

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