Abstract: Objective To investigate the protective mechanism of hydrogen sulfide (H2S) on rat gastric mucosal subjected to ischemia and perfusion. Methods Celiac artery of rates was clamped for 30 min and followed by 1 h reperfusion to induce gastric ischmia-reperfusion (GI-R) . Gastric mucosal damage was analyzed with macroscopic injured area. The expression of Bcl-2, Bax and caspase-3 in the gastric mucosal were determined by western blotting. Results Pretreatment of NaHS ( H2S exogenous donor) significantly reduced the g astric mucosal damage area induce by GI-R. Meanwhile, NaHS pretreatment inhibited the cellular Bcl-2, Bax and caspase-3 expression compared with GI-R group. Conclusion NaHS could attenuate GI-R injury . And the protective effect of exogenous H2S possibly through inh ibiting the pathway of gastric mucosal apoptosis.