Abstract: objective The differentially expressed genes (DEGs) in the inner ear of rats with chronic intrauterine hypoxia (CIH) were screened and analyzed by bioinformatics. Methods Twenty-four pregnant SD rats were randomly divided into normal group and anoxic group. After delivery, hearing test was performed on the normal hearing group (COH group) and deaf-induced rats (MH group). Result The purity of total experimental RNA extraction is qualified②There were 180 differentially expressed genes (old Change > 2 P value ≤ 0.05), 88 up-regulated genes and 92 down-regulated genes after CIH. GO functional annotation shows that in the molecular functional GO classification, the differential genes have voltage gated potassium channel activity, methylation CpG binding, active transmembrane transport activity, etc. in biological process, differential genes mainly participate in protein transport, oxygen transport and small molecule metabolism, and cell components are classified into GO classification: differentially genes are enriched in cell surface, receptors Complex, extracellular space, and intrinsic components of the membrane; pathway enrichment analysis showed that the differentially expressed genes were mainly more than 30 pathways of transcription disorders such as kappa B signaling pathway, JNK signaling pathway, NOD like receptor signaling pathway and Toll like receptor signal transduction pathway. ③Read-time PCR confirmed that the up-regulation or down-regulation of DEGs was basically consistent with the results of gene chip detection. Conclusion during pregnancy, CIH can up-regulate the apoptosis-promoting gene of MH group, such as Tsc22d3, Tsc22d3, Casp12, Dapk1, etc., down-regulate the ion balance gene Slc2a4, CLDN14, GJB6, aquaporin Aqp7, transcription factor POU4F3, etc. The down-regulation of the apoptosis suppressor gene, Birc3Nol3, Birc5, HellsPak 7 and so on, makes the κ B signaling pathway, JNK signal pathway and other transduction pathways transcriptional imbalance