Abstract: Objective To discuss the clinical analysis of rat nerve growth factor combined with Methylprednisolone in the treatment of sudden deafness (SD). Methods Seventy-eight patients with SD treated in our hospital from February 2015 to October 2017 were selected as our observational objects. They were randomly divided into a control group and an observation group. The control group were treated with vasodilation, nutritional nerves and Methylprednisolone intratympanic injection, and the observation group was added with intramuscular injection of rat nerve growth factor on the basis of the control group. The serum levels of vascular endothelial cells (VE-cadherin), vascular adhesion molecule-1 (sVCAM-1), high mobility protein-1 (HMGB1) levels, pure tone threshold and hearing impairment scores before and after treatment were compared between the two groups. The disappeared time of Symptoms was recorded. After 2 weeks of treatment, comprehensive clinical efficacy was evaluated and adverse events was recorded. Results Serum VE-cadherin, sVCAM-1 and HMGB1 levels significantly decreased in both groups after treatment, and the observation group was significantly lower than the control group after treatment (P<0.05); Pure tone threshold and hearing impairment score significantly decreased after treatment in both groups. And the observation group was significantly lower than the control group (P<0.05). The tinnitus, dizziness, and ear boring time in the observation group were significantly lower than those in the control group. The difference was statistically significant (P<0.05). The total effective rates of observation group and control group were 92.31% and 82.05%, respectively, and the difference was statistically significant (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion Rat nerve growth factor combined with Methylprednisolone can significantly improve the clinical symptoms of tinnitus and dizziness in SD patients, enhance hearing level and reduce the serum level of disease-related factors, with it being safe and effective.