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    向臣希, 刘慧, 李玉冰. miR-454-3p通过靶向RUNX3调控乳腺癌细胞的增殖、迁移和侵袭[J]. 徐州医科大学学报, 2020, 40(8): 559-564.
    引用本文: 向臣希, 刘慧, 李玉冰. miR-454-3p通过靶向RUNX3调控乳腺癌细胞的增殖、迁移和侵袭[J]. 徐州医科大学学报, 2020, 40(8): 559-564.
    shu
    miR-454-3p Targeting at RUNX3 to Regulate the Proliferation, Migration and Invasion Capacity of Breast Cancer Cells[J]. Journal of Xuzhou Medical University, 2020, 40(8): 559-564.
    Citation: miR-454-3p Targeting at RUNX3 to Regulate the Proliferation, Migration and Invasion Capacity of Breast Cancer Cells[J]. Journal of Xuzhou Medical University, 2020, 40(8): 559-564.
    shu

    miR-454-3p通过靶向RUNX3调控乳腺癌细胞的增殖、迁移和侵袭

    miR-454-3p Targeting at RUNX3 to Regulate the Proliferation, Migration and Invasion Capacity of Breast Cancer Cells

      摘要
    • 摘要: 目的 本研究探讨miR-454-3p对乳腺癌细胞增殖、迁移和侵袭的影响，及miR-454-3p是否靶向RUNX3。方法 在乳腺癌细胞MCF-7和T47D中转染miR-454-3p mimics和miR-454-3p inhibitor后，通过细胞克隆形成实验评估细胞的增殖能力；Transwell、细胞划痕实验评估细胞的迁移、侵袭的能力；Western blot 法检测其对蛋白RUNX3的调控作用。结果 与对照组相比，miR-454-3p mimics组乳腺癌细胞增殖、迁移和侵袭能力明显增高；miR-454-3p inhibitor组乳腺癌细胞增殖、迁移和侵袭能力明显下降。在乳腺癌细胞MCF-7和T47D中，miR-454-3p能够降低RUNX3蛋白的表达水平。结论miR-454-3p能够在体外靶向抑制RUNX3的表达而增加乳腺癌细胞的增殖、迁移和侵袭能力。

       

      Abstract: Purpose To investigate the effect of miR-454-3p on breast cancer cells(MCF-7,T47D) and whether miR-454-3p targets RUNX3. Methods After transfection of miR-454-3p mimics and miR-454-3p inhibitor into MCF-7 and T47D cells, cell proliferation ability was evaluated by colony formation assays, the invasion and migration capacity were assessed by wound-healing and Transwell assay s. Western blot assay was used to detect the protein level of RUNX3. Results Compared with the control group, miR-454-3p mimics significantly promoted the proliferation, migration and invasion capacity of MCF7 and T47D cells, w hereas miR-454-3p inhibitor remarkably inhibited those capacity in MCF7 and T47D cells . It was further confirmed that miR-454-3p mimics could facilitate the ex＜x＞pression of RUNX3, while miR-454-3p inhibitor inhibited it. Conclusions miR -454-3p could promote the proliferation, migration and invasion capacity of breast cancer cells by targeting at RUNX3

       

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