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    刘婷婷. ɑ-山竹黄酮对高糖培养的肾小球系膜细胞凋亡的影响[J]. 徐州医科大学学报, 2021, 41(8): 581-585.
    引用本文: 刘婷婷. ɑ-山竹黄酮对高糖培养的肾小球系膜细胞凋亡的影响[J]. 徐州医科大学学报, 2021, 41(8): 581-585.
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    The Effect of Alpha-mangostin on the Apoptosis in Mouse Mesangial Cells in High Glucose Medium[J]. Journal of Xuzhou Medical University, 2021, 41(8): 581-585.
    Citation: The Effect of Alpha-mangostin on the Apoptosis in Mouse Mesangial Cells in High Glucose Medium[J]. Journal of Xuzhou Medical University, 2021, 41(8): 581-585.
    shu

    ɑ-山竹黄酮对高糖培养的肾小球系膜细胞凋亡的影响

    The Effect of Alpha-mangostin on the Apoptosis in Mouse Mesangial Cells in High Glucose Medium

      摘要
    • 摘要: 目的：体外实验研究α-山竹黄酮通过抑制酸性鞘磷脂酶（ASMase）及内质网应激信号通路对高糖培养下的小鼠肾小球系膜细胞(MES13)凋亡的影响。方法：将传代培养的MES13细胞同步化后分组 ：（1）NG 组（正常浓度葡萄糖组，5.5mmol/L 葡萄糖）；（2）NG+a-M 组（正常浓度葡萄糖+α-山竹黄酮组）；（3）HG 组（高浓度葡萄糖组，30mmol/L）；（4）HG+低浓度 a-M 组（HG+1μM α-山竹黄酮）；（5）HG+中浓度a-M 组（HG+2μM α-山竹黄酮）；（6）HG+高浓度 a-M 组（HG+4μM α- 山竹黄酮）；（7）HG+des 组（HG+地西帕明）。各组细胞培养 24 小时，采用CCK-8法检测细胞活性；采用流式细胞术检测细胞凋亡情况；用 western blot 检测各组细胞ASMase及内质网应激相关蛋白GRP78、p-PERK、ATF4及内质网应激凋亡相关蛋白CHOP、caspase12 的表达水平。结果：1、与 NG 组相比，HG 组MES13 细胞凋亡水平明显增加（P<0.05）；与 HG 组相比，HG+a-M 组细胞凋亡率降低，且呈剂量依赖性减少；2、与 NG 组相比，HG 组MES13 细胞 ASMase 蛋白表达显著增加（P<0.05），与 HG 组相比，HG+a-M 组 ASMase 蛋白表达减少（P<0.05）；3、与 NG 组相比，HG 组 GRP78、p-PERK、ATF4、CHOP、caspase12 等蛋白表达水平显著增加（P<0.05）；与 HG 组相比，HG+a-M 组 GRP78、p-PERK、ATF4、CHOP、caspase12 等蛋白表达水平明显减少（P<0.05）。结论：α-山竹黄酮通过抑制高糖激活的ASMase，降低内质网应激信号通路，进而减少高糖培养的MES13细胞凋亡，为糖尿病肾病的治疗提供新的参考依据。

       

      Abstract: 0bjective To study the effect of alpha-mangostin on inhibiting acid sphingomyelinase （ASMase）and endoplasmic reticulum stress signaling pathway under high glucose in mouse mesangial cells (MES13) apoptosis．Methods Synchronized MES13 were divided into following groups：(1) NG group (normal concentration of glucose group, 5.5mmol/L glucose); (2)NG+ a-M group (normal concentration of glucose + alpha-mangostin group); (3) HG group (high concentration glucose group, 30mmol/L); (4)HG+ low concentration of a-M group (HG+1μM alpha-mangostin); (5)HG+ medium concentration a-M group (HG+2μM alpha-mangostin); (6)HG+ high concentration of a-M group (HG+4μM alpha-mangostin); (7)HG+des group (HG+ desipramine). Cells were cultured for 24 hours, and cell activity was detected by cck-8 assay. cell apoptosis was detected by flow cytometry. The protein ex＜x＞pressionlevels of ASMase, GRP78, p-PERK, ATF4, CHOP and caspase12 were detected by western blot. Results 1. Compared with NG group, the apoptosis level of MES13 cells in HG group was significantly increased (P<0.05). Compared with HG group, the apoptosis rate of HG+ a-M group was decreased. 2. Compared with NG group, the ex＜x＞pression of ASMase protein in MES13 cells in HG group was significantly increased (P<0.05), and the ex＜x＞pression of ASMase protein was decreased in HG+ a-M group compared with HG group (P<0.05). 3. Compared with NG group, the ex＜x＞pression levels of GRP78, p-PERK, ATF4, CHOP and caspase12 were significantly increased in HG group (P<0.05). Compared with HG group,the ex＜x＞pression levels of GRP78, p-PERK, ATF4, CHOP and caspase12 were significantly decreased in HG+ a-M group (P<0.05). Conclusion Alpha-mangostin may reduce the endoplasmic reticulum stress signal pathway by inhibiting the ASMase activated by high glucose, so as to alleviate the apoptosis of MES13 cells cultured in high glucose.This provides a new basis for the therapeutic target in the treatment of diabetic nephropathy

       

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