[1]李亚丰,李进冬,魏鹏翔,等. 大黄素对高糖诱导的大鼠肾小球系膜细胞TGF-β1/ERK1/2及FN蛋白表达的影响[J].徐州医科大学学报,2017,37(08):491-494.
 LI Yafeng,LI Jindong,WEI Pengxiang,et al. Effects of emodin on TGF-β1/ERK1/2 and FN protein expression in rat glomerular mesangial cells induced by high glucose[J].Journal of Xuzhou Medical University,2017,37(08):491-494.
点击复制

 大黄素对高糖诱导的大鼠肾小球系膜细胞TGF-β1/ERK1/2及FN蛋白表达的影响()
分享到:

《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
37
期数:
2017年08期
页码:
491-494
栏目:
出版日期:
2017-08-25

文章信息/Info

Title:
 Effects of emodin on TGF-β1/ERK1/2 and FN protein expression in rat glomerular mesangial cells induced by high glucose
文章编号:
2096-3882(2017)08-0491-04
作者:
 李亚丰1李进冬2魏鹏翔1吴静3宋知颖3王萌萌3渠珍珍3魏群利13*么焕开3
 1.江苏省新药研究与临床药学重点实验室,江苏徐州221004;2.泰州市人民医院药学部,江苏泰州225300;3.徐州医科大学药学院,江苏徐州221004
Author(s):
 LI Yafeng1 LI Jindong2 WEI Pengxiang1 WU Jing3 SONG Zhiying3 WANG Mengmeng3 QU Zhenzhen3 WEI Qunli13* YAO Huankai3
1.Key Laboratory of New Drug Research and Clinical Pharmacy of Jiangsu Province, Xuzhou, Jiangsu 221004, China;
2. Department of Pharmacy, the People′s Hospital of Taizhou,
Taizhou, Jiangsu 225300;
3. School of Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu 221004
关键词:
 大黄素高糖诱导肾小球系膜细胞糖尿病肾病增殖
Keywords:
 emodin high glucose-induced glomerular mesangial cells diabetic nephropathy proliferation
分类号:
R285.5
文献标志码:
A
摘要:
 目的探讨大黄素(emodin,EMO)对高糖诱导的大鼠肾小球系膜细胞(glomerular mesangial cells,MCs)转化生长因子-β1(TGF-β1)/细胞外信号调节激酶1/2(ERK1/2)及细胞外基质(extracellular matrix,ECM)FN蛋白表达的影响。方法将常规培养的MCs分为正常组(NG,5.56 mmol/L葡萄糖)、 甘露醇组(MA,5.56 mmol/L葡萄糖+24.44 mmol/L甘露醇)、溶剂组(0.1% DMSO+30 mmol/L葡萄糖)、高糖组(HG,30 mmol/L葡萄糖)及大黄素干预组(1 μmol/L、10 μmol/L EMO+30 mmol/L葡萄糖)。CCK-8检测细胞增殖情况;Western blotting检测各组细胞TGF-β1、p-ERK1/2、FN蛋白表达。结果与正常组比较,高糖培养48 h MCs增殖显著(P<0.05,P<0.01),p-ERK1/2、TGF-β1、FN蛋白表达明显增高(P<0.05);与高糖组比较,大黄素干预组能抑制MCs增殖,并显著降低p-ERK1/2、TGF-β1、FN蛋白表达(P<0.05或P<0.01)。结论大黄素能抑制高糖诱导下的MCs细胞增殖,下调TGF-β1、p-ERK1/2、FN蛋白表达,从而发挥对糖尿病肾病(diabetic nephropathy,DN)的防治作用。
Abstract:
 ObjectiveTo investigate the effects of emodin (EMO) on the TGF-β1/ERK1/2, extracellular matrix (ECM) and FN protein in rat glomerular mesangial cells (MCs) induced by high glucose. MethodsMCs were divided into four groups: a normal control group (NG, 5.56 mmol/L glucose), a mannitol group (MA, 5.56 mmol/L+24.44 mmol/L mannitol), a solvent group (0.1%DMSO+30 mmol/L glucose), a high glucose group (HG, 30 mmol/L glucose) and an Emodin intervention group (1 μmol/L, 10 μmol/L EMO+30 mmol/L glucose). The cell proliferation was measured by CCK-8 assay. The levels of TGF-β1, p-ERK1/2 and FN were detected by Western blotting. ResultsCompared with the NG group, the HG group presented obvious proliferation after cultivation for 48 h (P<0.05 or P<0.01) and produced significantly increased levels of p-ERK1/2, TGF-β1 and FN (P<0.05). Compared with the HG group, the Emodin intervention group showed suppressed proliferation and remarkably reduced levels of p-ERK1/2, TGF-β1, and FN (P<0.05 or P<0.01). ConclusionsEmodin can inhibit the proliferation of MCs induced by high glucose and down-regulate the levels of p-ERK1/2, TGF-β1 and FN, which plays a role in the prevention and treatment of diabetic nephropathy.

参考文献/References:

 1]Forbes JM, Cooper ME. Mechanisms of diabetic complications [J]. Physiol Rev, 2013,93(1):137-188.
[2]Fox CS, Larson MG, Leip EP, et al. Glycemic status and development of kidney disease: the Framingham Heart Study [J]. Diabetes Care, 2005,28(10):2436-2440.
[3]Kanwar YS, Wada J, Sun L, et al. Diabetic nephropathy: mechanisms of renal disease progression [J]. Exp Biol Med (Maywood), 2008,233(1):4-11.
[4]Han DC, Hoffman BB, Hong SW, et al. Therapy with antisense TGF-β1 oligodeoxynucleotides reduces kidney weight and matrix mRNAs in diabetic mice[J]. Am J Physiol Renal Physiol, 2000,278(4):F628-F634.
[5]Robinson MJ, Cobb MH. Mitogen-activated protein kinase pathways[J]. Curr Opin Cell Biol, 1997,9(2):180-186.
[6]Liu S, Liang Y, Huang H, et al. ERK-dependent signaling pathway and transcriptional factor Ets-1 regulate matrix metalloproteinase-9 production in transforming growth factor-β1 stimulated glomerular podocytes [J]. Cell Physiol Biochem, 2005,16(4-6):207-216.
[7]Huang H, Huang H, Ying L, et al. Gremlin induces cell proliferation and extra cellular matrix accumulation in mouse mesangial cells exposed to high glucose via the ERK1/2 pathway [J/OL]. BMC Nephrol, 2013,14(1):33. doi:10.1186/1471-2369-14-33.
[8]Wang J, Fang H, Dong B, et al. Effects of free anthraquinones extract from the rhubarb on cell proliferation and accumulation of extracellular matrix in high glucose cultured-mesangial cells [J]. Korean J Physiol Pharmacol, 2015,19(6):485-489.
[9]Yang J, Zeng Z, Wu T, et al. Emodin attenuates high glucose-induced TGF-β1 and fibronectin expression in mesangial cells through inhibition of NF-κB pathway [J]. Exp Cell Res, 2013,319(20):3182-3189.
[10]Takamiya Y, Fukami K, Yamagishi S, et al. Experimental diabetic nephropathy is accelerated in matrix metalloproteinase-2 knockout mice [J]. Nephrol Dial Transplant, 2013,28(1):55-62.
[11]李慎军,张忠辉.糖尿病肾病基底膜成分的改变[J].国外医学·内分泌学分册,2000,20(3):132-135.
[12]Hayama M, Akiba S, Fukuzumi M, et al. High glucose-induced cytosolic phospholipase A2 activation responsible for eicosanoid production in rat mesangial cells [J]. J Biochem, 1997,122(6):1196-1201.
[13]周晖,古继红,莫伟,等.滋水分清饮治疗早期糖尿病肾病20例疗效观察[J].新中医,2010,42(7):23-24.
[14]李慧,曹文富,汤为学,等.解聚复肾宁对大鼠肾小球系膜细胞增殖和细胞外基质分泌的影响[J].中成药,2010,32(6):918-921.
[15]张志忠,王彩霞,魏建红,等.益气养阴合剂治疗糖尿病肾病临床研究[J].中华中医药学刊.2009,27(8):1755-1757.

相似文献/References:

[1]孙秀媛,李海玲,朱相华,等.rTMS及MECT在不同时间点对抑郁症疗效和BDNF的影响及相关性研究[J].徐州医科大学学报,2017,37(04):262.
[2]代春潇,宋远见,张云山,等. 大鼠MKK7真核表达质粒的构建及其在COS7细胞中转染效率[J].徐州医科大学学报,2017,37(04):211.
  [J].Journal of Xuzhou Medical University,2017,37(08):211.
[3]张端强,丁晓帆,杨丹丹,等. 加综合征患者复发影响因素研究[J].徐州医科大学学报,2017,37(04):216.
 [J].Journal of Xuzhou Medical University,2017,37(08):216.
[4]王立新,袁峰*,郭开今,等. 纳米羟基磷灰石/细菌纤维素对兔骨髓间充质干细胞黏附及成骨分化的影响[J].徐州医科大学学报,2017,37(04):221.
 [J].Journal of Xuzhou Medical University,2017,37(08):221.
[5]李海青,曹猛,江涛,等. PD-L1在结直肠癌中的表达及其临床意义[J].徐州医科大学学报,2017,37(04):227.
 [J].Journal of Xuzhou Medical University,2017,37(08):227.
[6]刘兵,唐明生,李苹,等. 用随机森林模型分析胃癌高危人群干预效果的影响因素*[J].徐州医科大学学报,2017,37(04):231.
 [J].Journal of Xuzhou Medical University,2017,37(08):231.
[7]周新宇?,牛坚,曹宽,等. Tim3在炎症反应调控及对肝细胞损伤保护机制研究[J].徐州医科大学学报,2017,37(04):236.
 [J].Journal of Xuzhou Medical University,2017,37(08):236.
[8]商书霞,宋光耀*,刘晓耕,等. 血清胆红素和γ-谷氨酰转肽酶与糖尿病患者非酒精性脂肪肝的相关性研究[J].徐州医科大学学报,2017,37(04):245.
 [J].Journal of Xuzhou Medical University,2017,37(08):245.
[9]李新文1,巩尊科2,王世雁2,等. RS在非痴呆型血管性认知障碍评定中的临床应用研究[J].徐州医科大学学报,2017,37(04):248.
 [J].Journal of Xuzhou Medical University,2017,37(08):248.
[10]樊叶,韩维维,何帮顺,等.替格瑞洛含药血清对人胃黏膜上皮细胞GES-1的损伤作用[J].徐州医科大学学报,2017,37(04):252.
 [J].Journal of Xuzhou Medical University,2017,37(08):252.

备注/Memo

备注/Memo:
 基金项目:国家自然科学基金(81302744)
*通信作者,E-mail:weiqunli@126.com
更新日期/Last Update: 2017-09-05