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    杨俊霞, 花璐, 姜彦羽, 韩文灿, 李燕强. 突触结合蛋白1在神经病理性疼痛小鼠前扣带回表达的变化[J]. 徐州医科大学学报, 2017, 37(11): 707-710.
    引用本文: 杨俊霞, 花璐, 姜彦羽, 韩文灿, 李燕强. 突触结合蛋白1在神经病理性疼痛小鼠前扣带回表达的变化[J]. 徐州医科大学学报, 2017, 37(11): 707-710.
    YANG Junxia, HUA Lu, JIANG Yanyu, HAN Wencan, LI Yanqiang. A study of the changes of synaptotagmin 1 in the anterior cingulate cortex of mice with neuropathic pain[J]. Journal of Xuzhou Medical University, 2017, 37(11): 707-710.
    Citation: YANG Junxia, HUA Lu, JIANG Yanyu, HAN Wencan, LI Yanqiang. A study of the changes of synaptotagmin 1 in the anterior cingulate cortex of mice with neuropathic pain[J]. Journal of Xuzhou Medical University, 2017, 37(11): 707-710.

    突触结合蛋白1在神经病理性疼痛小鼠前扣带回表达的变化

    A study of the changes of synaptotagmin 1 in the anterior cingulate cortex of mice with neuropathic pain

    • 摘要: 目的探讨突触结合蛋白1(synaptotagmin 1,Syt1)在神经病理性疼痛小鼠前扣带回表达的变化及与痛行为的关系。方法将20~25 g的小鼠随机分为4组:假手术(Sham)+Syt1抗体(Syt1-Ab)组,Sham+对照抗体(Con-IgG)组,坐骨神经结扎(CCI)+Syt1-Ab组,CCI+Con-IgG组。在CCI第7天给每组小鼠前扣带回立体定位注射相应药物(0.1 μg/0.5 μl),按Hargreaves法用热辐射刺激仪测定小鼠热痛的变化,用电子测痛仪测定小鼠机械痛缩足阈值的变化,免疫印迹法检测各组小鼠前扣带回Syt1的表达。结果免疫印迹显示神经病理性疼痛小鼠前扣带回Syt1表达明显升高,在神经病理性疼痛小鼠前扣带回立体定位注射Syt1-Ab可明显改善痛行为。结论神经病理性疼痛小鼠前扣带回Syt1表达显著升高,在神经病理性疼痛小鼠前扣带回立体定位注射Syt1-Ab可缓解疼痛。

       

      Abstract: ObjectiveTo investigate the expression of synaptotagmin 1 (Syt 1) in the anterior cingulate cortex of mice with neuropathic pain and its relationship with pain behavior. MethodsA total of mice weighting 20-25 g were randomly divided into four groups: Group Sham+Syt antibody (Syt-Ab), Group Sham+Control-IgG (Con-IgG), Group chronic constriction injury (CCI)+Syt-Ab and Group CCI+Con-IgG. Corresponding drugs (0.1 μg/0.5 μl) were stereotaxically injected into the anterior cingulate cortex of mice in each group on Day 7 of CCI. The changes of thermal hyperalgesia were measured by the Hargreaves method. The changes of mechanical paw withdrawal threshold were determined by an electronic dolorimeter. The level of Syt 1 in the anterior cingulate cortex of each group was detected by Western blotting. ResultsThe amount of Syt 1 was remarkably increased in the anterior cingulate cortex of mice with neuropathic pain. Stereotactic injection of Syt 1 Ab could significantly improve pain behavior. ConclusionsThe expression of Syt 1 is significantly increased in the anterior cingulate cortex of mice with neuropathic pain. The pain can be relieved by stereotaxic injection of Syt 1 Ab in the anterior cingulate cortex.

       

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