[1]杨俊霞,花璐,姜彦羽,等.突触结合蛋白1在神经病理性疼痛小鼠前扣带回表达的变化[J].徐州医科大学学报,2017,37(11):707-710.
　YANG Junxia,HUA Lu,JIANG Yanyu,et al.A study of the changes of synaptotagmin 1 in the anterior cingulate cortex of mice with neuropathic pain[J].Journal of Xuzhou Medical University,2017,37(11):707-710.
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突触结合蛋白1在神经病理性疼痛小鼠前扣带回表达的变化()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
37
期数:
2017年11期
页码:
707-710
栏目:
出版日期:
2017-11-25

文章信息/Info

Title:
A study of the changes of synaptotagmin 1 in the anterior cingulate cortex of mice with neuropathic pain
作者:
杨俊霞1; 花璐2; 姜彦羽3; 韩文灿1; 李燕强1*
1.徐州医科大学江苏省麻醉学重点实验室，江苏省麻醉与镇痛应用技术重点实验室，江苏徐州221004； 2.华中科技大学同济医学院附属同济医院麻醉科，湖北武汉430030； 3.徐州医科大学附属医院麻醉科，江苏徐州221002
Author(s):
YANG Junxia1;  HUA Lu2;  JIANG Yanyu3;  HAN Wencan1;  LI Yanqiang1*
1.Jiangsu Province Key Laboratory of Anesthesiology, Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China; 2.Department of Anesthesiology, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei 430030; 3.Department of Anesthesiology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002
关键词:
突触结合蛋白1; 神经病理性疼痛; 前扣带回
Keywords:
synaptotagmin 1;  neuropathic pain;  anterior cingulate cortex
分类号:
R614.2
文献标志码:
A
摘要:
目的探讨突触结合蛋白1（synaptotagmin 1，Syt1）在神经病理性疼痛小鼠前扣带回表达的变化及与痛行为的关系。方法将20～25 g的小鼠随机分为4组：假手术（Sham）+Syt1抗体（Syt1-Ab）组，Sham+对照抗体（Con-IgG）组，坐骨神经结扎(CCI)+Syt1-Ab组，CCI+Con-IgG组。在CCI第7天给每组小鼠前扣带回立体定位注射相应药物（0.1 μg/0.5 μl），按Hargreaves法用热辐射刺激仪测定小鼠热痛的变化，用电子测痛仪测定小鼠机械痛缩足阈值的变化，免疫印迹法检测各组小鼠前扣带回Syt1的表达。结果免疫印迹显示神经病理性疼痛小鼠前扣带回Syt1表达明显升高，在神经病理性疼痛小鼠前扣带回立体定位注射Syt1-Ab可明显改善痛行为。结论神经病理性疼痛小鼠前扣带回Syt1表达显著升高，在神经病理性疼痛小鼠前扣带回立体定位注射Syt1-Ab可缓解疼痛。
Abstract:
ObjectiveTo investigate the expression of synaptotagmin 1 (Syt 1) in the anterior cingulate cortex of mice with neuropathic pain and its relationship with pain behavior. MethodsA total of mice weighting 20-25 g were randomly divided into four groups: Group Sham+Syt antibody (Syt-Ab), Group Sham+Control-IgG (Con-IgG), Group chronic constriction injury (CCI)+Syt-Ab and Group CCI+Con-IgG. Corresponding drugs (0.1 μg/0.5 μl) were stereotaxically injected into the anterior cingulate cortex of mice in each group on Day 7 of CCI. The changes of thermal hyperalgesia were measured by the Hargreaves method. The changes of mechanical paw withdrawal threshold were determined by an electronic dolorimeter. The level of Syt 1 in the anterior cingulate cortex of each group was detected by Western blotting. ResultsThe amount of Syt 1 was remarkably increased in the anterior cingulate cortex of mice with neuropathic pain. Stereotactic injection of Syt 1 Ab could significantly improve pain behavior. ConclusionsThe expression of Syt 1 is significantly increased in the anterior cingulate cortex of mice with neuropathic pain. The pain can be relieved by stereotaxic injection of Syt 1 Ab in the anterior cingulate cortex.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金青年基金（81500950）；徐州市科技计划项目（KC14SH079） *通信作者，E-mail：yjx001011@163.com
更新日期/Last Update: 2017-12-06