[1]杨俊霞,花璐,姜彦羽,等.突触结合蛋白1在神经病理性疼痛小鼠前扣带回表达的变化[J].徐州医科大学学报,2017,37(11):707-710.
 YANG Junxia,HUA Lu,JIANG Yanyu,et al.A study of the changes of synaptotagmin 1 in the anterior cingulate cortex of mice with neuropathic pain[J].Journal of Xuzhou Medical University,2017,37(11):707-710.
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突触结合蛋白1在神经病理性疼痛小鼠前扣带回表达的变化()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
37
期数:
2017年11期
页码:
707-710
栏目:
出版日期:
2017-11-25

文章信息/Info

Title:
A study of the changes of synaptotagmin 1 in the anterior cingulate cortex of mice with neuropathic pain
作者:
杨俊霞1花璐2姜彦羽3韩文灿1李燕强1*
1.徐州医科大学江苏省麻醉学重点实验室,江苏省麻醉与镇痛应用技术重点实验室,江苏徐州221004; 2.华中科技大学同济医学院附属同济医院麻醉科,湖北武汉430030; 3.徐州医科大学附属医院麻醉科,江苏徐州221002
Author(s):
YANG Junxia1 HUA Lu2 JIANG Yanyu3 HAN Wencan1 LI Yanqiang1*
1.Jiangsu Province Key Laboratory of Anesthesiology, Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China; 2.Department of Anesthesiology, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei 430030; 3.Department of Anesthesiology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002
关键词:
突触结合蛋白1神经病理性疼痛前扣带回
Keywords:
synaptotagmin 1 neuropathic pain anterior cingulate cortex
分类号:
R614.2
文献标志码:
A
摘要:
目的探讨突触结合蛋白1(synaptotagmin 1,Syt1)在神经病理性疼痛小鼠前扣带回表达的变化及与痛行为的关系。方法将20~25 g的小鼠随机分为4组:假手术(Sham)+Syt1抗体(Syt1-Ab)组,Sham+对照抗体(Con-IgG)组,坐骨神经结扎(CCI)+Syt1-Ab组,CCI+Con-IgG组。在CCI第7天给每组小鼠前扣带回立体定位注射相应药物(0.1 μg/0.5 μl),按Hargreaves法用热辐射刺激仪测定小鼠热痛的变化,用电子测痛仪测定小鼠机械痛缩足阈值的变化,免疫印迹法检测各组小鼠前扣带回Syt1的表达。结果免疫印迹显示神经病理性疼痛小鼠前扣带回Syt1表达明显升高,在神经病理性疼痛小鼠前扣带回立体定位注射Syt1-Ab可明显改善痛行为。结论神经病理性疼痛小鼠前扣带回Syt1表达显著升高,在神经病理性疼痛小鼠前扣带回立体定位注射Syt1-Ab可缓解疼痛。
Abstract:
ObjectiveTo investigate the expression of synaptotagmin 1 (Syt 1) in the anterior cingulate cortex of mice with neuropathic pain and its relationship with pain behavior. MethodsA total of mice weighting 20-25 g were randomly divided into four groups: Group Sham+Syt antibody (Syt-Ab), Group Sham+Control-IgG (Con-IgG), Group chronic constriction injury (CCI)+Syt-Ab and Group CCI+Con-IgG. Corresponding drugs (0.1 μg/0.5 μl) were stereotaxically injected into the anterior cingulate cortex of mice in each group on Day 7 of CCI. The changes of thermal hyperalgesia were measured by the Hargreaves method. The changes of mechanical paw withdrawal threshold were determined by an electronic dolorimeter. The level of Syt 1 in the anterior cingulate cortex of each group was detected by Western blotting. ResultsThe amount of Syt 1 was remarkably increased in the anterior cingulate cortex of mice with neuropathic pain. Stereotactic injection of Syt 1 Ab could significantly improve pain behavior. ConclusionsThe expression of Syt 1 is significantly increased in the anterior cingulate cortex of mice with neuropathic pain. The pain can be relieved by stereotaxic injection of Syt 1 Ab in the anterior cingulate cortex.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金青年基金(81500950);徐州市科技计划项目(KC14SH079) *通信作者,E-mail:yjx001011@163.com
更新日期/Last Update: 2017-12-06