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    黄成娅, 方昕, 李春霖, 陈施吾, 吴金霞. 雷帕霉素和氯喹对急性酒精损伤后蟾蜍心脏活动的影响[J]. 徐州医科大学学报, 2017, 37(11): 711-714.
    引用本文: 黄成娅, 方昕, 李春霖, 陈施吾, 吴金霞. 雷帕霉素和氯喹对急性酒精损伤后蟾蜍心脏活动的影响[J]. 徐州医科大学学报, 2017, 37(11): 711-714.
    HUANG Chengya, FANG Xin, LI Chunlin, CHEN Shiwu, WU Jinxia. Effects of rapamycin and chloroquine on the heart of toads after acute alcoholic injury[J]. Journal of Xuzhou Medical University, 2017, 37(11): 711-714.
    Citation: HUANG Chengya, FANG Xin, LI Chunlin, CHEN Shiwu, WU Jinxia. Effects of rapamycin and chloroquine on the heart of toads after acute alcoholic injury[J]. Journal of Xuzhou Medical University, 2017, 37(11): 711-714.

    雷帕霉素和氯喹对急性酒精损伤后蟾蜍心脏活动的影响

    Effects of rapamycin and chloroquine on the heart of toads after acute alcoholic injury

    • 摘要: 目的探讨雷帕霉素和氯喹对急性酒精损伤后蟾蜍心脏活动的影响。方法将45只蟾蜍分为雷帕霉素组(Rap组)、氯喹组(CQ组)和任氏液组(RS组),每组15只。采用心脏浸润法,对离体蟾蜍心脏给予浓度为60%的酒精造成急性心肌损伤,静置2 min后,用任氏液清洗。再行离体心脏灌流给药,分别给予任氏液、雷帕霉素0.1 g/L及氯喹0.2 g/L处理,20 min后心脏再次酒精浸润,于每次给药前后分别记录3组蟾蜍的心率和心肌收缩力。结果和第1次酒精损伤相比,第2次酒精造成的心脏损伤更为明显(P<0.05),且心率变化比心肌收缩力变化显著;与RS组第2次酒精损伤相比,CQ组心率及心肌收缩力损伤较为显著(P<0.01),而Rap组则明显改善(P<0.05)。结论酒精造成的蟾蜍心脏急性损伤与酒精浸润次数有关。氯喹作用后可加重心脏损伤,而雷帕霉素可改善酒精中毒造成的心脏损伤。 关键词:雷帕霉素;氯喹;酒精损伤;心脏;蟾蜍;自噬

       

      Abstract: ObjectiveTo explore the effects of rapamycin and chloroquine on the heart of toads after acute alcoholic injury. MethodsThe toads were randomly divided into a rapamycin group (Rap), a chloroquine group (CQ) and a Ringer’s group (RS) (n=15). An acute myocardial injury of toads was established using isolated hearts exposed to 60% alcohol for 2 min followed by being washed with Ringer’s solution. Then, the hearts were infused with 0.1 g/L rapamycin, 0.2 g/L chloroquine or the equal volumes of Ringer’s solution over 20 min before being infused within alcohol. The heart rate and contractility were detected before and after administration. ResultsMyocardial injury induced by alcohol was more obvious at the second time than that at the first time (P<0.05). The heart rate and myocardial contractility revealed an obvious decrease in the chloroquine treatment group compared with the control group (P<0.01), while pretreatment with rapamycin improved the heart rate and myocardial contractility of toads after acute alcoholic injury (P<0.05). ConclusionThe severity of myocardial injury caused by alcohol is correlated to its frequency and infusion. Chloroquine can cause aggravated myocardial damage, while rapamycin improves cardiac function.

       

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