Abstract: ObjectiveTo investigate the specific effects of pannexin1 on rats with myocardial ischemia reperfusion injury. MethodsThe expression of pannexin1 in rat heart was determined by immunohistochemical method. Then, in vitro and in vivo cardiac ischemia reperfusion models of rats were employed to determine the hemodynamic changes, coronary outflow at each time point of perfusion and the content of serum lactic dehydrogenase (LDH). The amount of formazan in isolated myocardial tissues was measured to determine myocardial activities. The myocardial infracted area was detected by TTC staining. ResultsPannexin1 were mainly expressed in ventricular myocytes. Compared with the group, probenecid, the inhibitor of pannexin1 significantly improved left ventricular end diastolic pressure, enhanced the content of formazan in isolated myocardial tissues, reduced the myocardial infarct area induced by ischemia perfusion and decreased the levels of LDH both in vitro and in vivo. ConclusionPannexin 1 is expressed in ventricular tissue and plays a role in myocardial ischemia reperfusion. Specific blockage of pannexin1 attenuates myocardial injury induced by ischemia reperfusion, which plays a protective role in myocardium.