[1]赵晗,金培生*. RUNX2基因对于ADSCs向表皮分化的影响[J].徐州医科大学学报,2017,37(05):287-292.
 ZHAO Han,JIN Peisheng*. Effects of RUNX2 gene on the epidermal differentiation of ADSCs[J].Journal of Xuzhou Medical University,2017,37(05):287-292.
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 RUNX2基因对于ADSCs向表皮分化的影响()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
37
期数:
2017年05期
页码:
287-292
栏目:
出版日期:
2017-05-25

文章信息/Info

Title:
 Effects of RUNX2 gene on the epidermal differentiation of ADSCs
作者:
 赵晗1金培生2*
 1.徐州医科大学研究生学院2014级,江苏徐州221004;2.徐州医科大学附属医院整形外科,江苏徐州221002
Author(s):
 ZHAO Han1 JIN Peisheng2*
 1.Grade 2014, Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China;
2.Department of Plastic Surgery, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002
关键词:
 人脂肪干细胞表皮分化RUNX2慢病毒创面修复
Keywords:
 human adipose derived stem cellsepidermal differentiationRUNX2lentivirus wound repair
分类号:
R622
文献标志码:
A
摘要:
目的 诱导人脂肪干细胞(adipose-derived stem cells, ADSCs)在体外向表皮细胞分化并探讨 RUNX2基因对 ADSCs 表皮分化的影响。方法 利用胶原酶消化法分离培养 ADSCs,体外诱导 ADSCs向表皮细胞分化。慢病毒载体介导RUNX2基因转染ADSCs并通过 Western blot、反转录聚合酶链反应(RT-PCR)及免疫荧光验证RUNX2、相关表皮细胞标记物CK18、ZO-1的蛋白和 mRNA的表达。结果 ADSCs向表皮诱导 14天后, RUNX2、CK18、ZO-1的 mRNA及蛋白的表达水平均明显高于对照组(P<0.05);过表达RUNX2慢病毒转染后的 ADSCs以上指标均显著高于对照组和阴性对照组(P<0.05)。结论 RUNX2过表达可在一定程度上促进 ADSCs向表皮细胞诱导分化。
Abstract:
 Objective To induce the differentiation of human adipose derived stem cells (ADSCs) into epidermal cells in vitro and to discuss the effects of RUNX2 gene on the epidermal differentiation of ADSCs. Methods ADSCs were isolated by collagenase digestion. Then, the cells obtained were induced to differentiate into epidermal cells in vitro and transfected with lentiviral vector-mediated RUNX2 gene. The expression of RUNX2, CK18, ZO-1 protein and mRNA was examined by Western blot, RT-PCR and immunofluorescence. Results After induction for 14 days, the levels of RUNX2, CK18 and ZO-1 mRNA and protein in ADSCs were remarkably higher than those in the control group (P<0.05). The ADSCs transfected with lentiviral vector-mediated RUNX2 produced higher amounts of RUNX2, CK18 and ZO-1 mRNA and protein than those in the control group and negative control group (P<0.05). Conclusion Overexpression of RUNX2 gene can stimulate epidermal differentiation of ADSCs to some extent.

参考文献/References:

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备注/Memo

备注/Memo:
 国家自然科学基金(81571901)
*通信作者,E-mail:Jinps2006@163.com
更新日期/Last Update: 2017-06-09