[1]高一淋,蔡红星*,郭开今. AQP4、AQP9在兔骨性关节炎软骨病变中的表达[J].徐州医科大学学报,2017,37(05):304-307.
　GAO Yilin,CAI Hongxing*,GUO Kaijin. The expression of AQP4 and AQP9 in the cartilage lesion of rabbits with knee osteoarthritis[J].Journal of Xuzhou Medical University,2017,37(05):304-307.
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 AQP4、AQP9在兔骨性关节炎软骨病变中的表达
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
37
期数:
2017年05期
页码:
304-307
栏目:
出版日期:
2017-05-25

文章信息/Info

Title:
 The expression of AQP4 and AQP9 in the cartilage lesion of rabbits with knee osteoarthritis
作者:
 高一淋1; 蔡红星2*; 郭开今3
1.徐州医科大学研究生学院2014级，江苏徐州221004；2.徐州医科大学法医学教研室，江苏徐州 221004；
3.徐州医科大学附属医院骨外科，江苏徐州221002
Author(s):
GAO Yilin1 ;  CAI Hongxing2*;  GUO Kaijin3
1.Grade 2014, Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China;
2.Teaching and Research Section of Forensic Medicine, Xuzhou Medical University;
3.Department of Orthopedic Surgery, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002
关键词:
 ; 骨性关节炎; 关节; 软骨; AQP4; AQP9; 兔
Keywords:
osteoarthritis;  articular;  cartilage;  AQP4;  AQP9;  rabbit
分类号:
r684.3
文献标志码:
A
摘要:
目的通过观察水通道蛋白4、水通道蛋白9（AQP4、AQP9）在膝骨性关节炎兔模型的膝关节软骨中的表达，从而探讨AQP4、AQP9 在膝骨性关节炎发生发展过程中的作用。方法采用兔膝关节伸直位制动膝骨性关节炎（OA）模型，取制动2、4、6、8和10周的兔膝关节股骨髁软骨组织制作病理切片，在光学显微镜下观察病理改变，并应用免疫组化检测各组关节软骨中AQP4、AQP9的表达。结果随着造模时间延长，兔膝关节软骨破坏程度不断加深；免疫组化染色显示膝骨性关节炎模型兔的膝软骨各时期AQP4、AQP9的表达均高于空白对照组，并且差异有统计学意义（P＜0.01）。结论AQP4、AQP9在膝骨性关节炎软骨中高度表达，推测其可能影响骨性关节炎的发生发展。
Abstract:
ObjectiveTo investigate the expression of aquaporin 4 (AQP4) and aquaporin 9 (AQP9) in the knee articular cartilage of rabbits with knee osteoarthritis (OA), so as to discuss the roles of AQP4 and AOP9 in the pathogenesis of knee osteoarthritis. MethodsThe right hind limbs of rabbits were immobilized with a plaster cast in the extension position to establish an OA model. The rabbits were sacrificed after 2, 4, 6, 8 and 10 weeks and their femoral condyle cartilage were taken to prepare histopathology sections for optical microscopic observation. ResultsThe damages in rabbit cartilages were worsen as the modeling process extended. According to immunohistochemistry, the expression of AQP4 and AQP9 were higher in the knee cartilage of the experimental groups than that of the control group, with statistical difference (P<0.01). ConclusionsAQP4 and AQP9 are highly expressed in the knee cartilage of OA, which indicates that AQP4 and AQP9 may be associated with the pathogenesis of OA.

参考文献/References:

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备注/Memo:
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更新日期/Last Update: 2017-06-09