[1]葛明明,刘福民.miR-451a通过MIF调节滋养细胞侵袭能力[J].徐州医科大学学报,2019,39(08):567-570.
 miR-451a regulates trophoblast invasion via MIF[J].Journal of Xuzhou Medical University,2019,39(08):567-570.
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miR-451a通过MIF调节滋养细胞侵袭能力()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
39
期数:
2019年08期
页码:
567-570
栏目:
出版日期:
2019-08-25

文章信息/Info

Title:
miR-451a regulates trophoblast invasion via MIF
作者:
葛明明刘福民
文献标志码:
A
摘要:
目的:探讨 miR-451a对人绒毛外滋养细胞侵袭能力的调节机制。方法 以人工合成的寡核苷酸miR-451a模拟物(miR-451a mimics)转染人绒毛外滋养细胞株HTR-8/SVneo细胞,应用逆转录-实时PCR方法检测转染后细胞中miR-451a的表达和MIF mRNA表达的变化;应用Western Blot方法检测转染后细胞中MIF蛋白表达的变化;应用Transwell小室侵袭实验观察转染前后细胞侵袭能力的变化;分别向HTR-8/SVneo细胞中加入重组人巨噬细胞迁移抑制因子(rhMIF)和MIF特异性抑制剂ISO-1,应用Western Blot方法检细胞中E-cadherin蛋白和Vimentin蛋白表达的变化。结果 转染miR-451a mimics后HTR-8/SVneo细胞中miR-451a表达明显升高,MIF mRNA及蛋白表达降低,细胞侵袭能力下降。加入不同药物后,与空白组相比,rhMIF组的E-cadherin蛋白表达下降,Vimentin蛋白表达上升;ISO-1组的E-cadherin蛋白表达上升,Vimentin蛋白表达下降。 结论 转染miR-451a可以下调人绒毛外滋养细胞中MIF的表达,并可能通过上皮间质转化途径调控人绒毛外滋养细胞的侵袭能力。
Abstract:
Objectives: To investigate the regulatory mechanism of miR-451a on human villous trophoblast invasion. Methods:Human chorionic villus trophoblast cell line HTR-8/SVneo was transfected with synthetic oligonucleotide miR-451a mimics. Reverse transcription-real-time PCR was used to detect the expression of miR-451a and the expression of MIF mRNA. The expression of MIF protein in transfected cells was detected by Western Blot. Transwell cell invasion assay was used to observe the changes of cell invasiveness before and after transfection. The recombinant human macrophage migration inhibitory factor (rhMIF) and ISO-1,the Specific inhibitors of MIF ,were added to HTR-8/SVneo cells. The expression of E-cadherin protein and Vimentin protein in cells was detected by Western Blot. Results:The miR-451a expression in HTR-8/SVneo cells transfected with miR-451a mimics was significantly increased, the expression of MIF mRNA and protein was decreased, and the cell invasive ability was decreased. Compared with the blank group, E-cadherin protein expression decreased and Vimentin protein expression increased in rhMIF group.The expression of E-cadherin protein expression increased and Vimentin protein expression decreased in ISO-1 group. Conclusion: Transfection of miR-451a down-regulates the expression of MIF in human extravillous trophoblast cells and may regulate the invasion ability of human villous trophoblast cells through the epithelial-mesenchymal transition pathway.
更新日期/Last Update: 2019-09-18