[1]曹春萍,郑哲,左冬姣,等.HIV-1 Tat蛋白激活AKT信号调节肝细胞脂肪代谢[J].徐州医科大学学报,2018,38(11):701-705.
　CAO Chunping,ZHENG Zhe,ZUO Dongjiao,et al.HIV-1 Tat enhances lipid metabolism in human hepatocytes via activating AKT signaling[J].Journal of Xuzhou Medical University,2018,38(11):701-705.
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HIV-1 Tat蛋白激活AKT信号调节肝细胞脂肪代谢()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
38
期数:
2018年11期
页码:
701-705
栏目:
出版日期:
2018-11-25

文章信息/Info

Title:
HIV-1 Tat enhances lipid metabolism in human hepatocytes via activating AKT signaling
作者:
曹春萍1; 2; 郑哲3; 左冬姣1; 高林1; 曹倩文1; 孔凡运1; 刘晓梅1; 汤仁仙1; 薛敏4; 周峰1*
1.徐州医科大学江苏省免疫与代谢重点实验室，江苏 徐州 221004；2.徐州市中心医院检验科，江苏 徐州 221009；3.徐州医科大学生物学系；4.徐州医科大学生理学教研室
Author(s):
CAO Chunping1; 2;  ZHENG Zhe3;  ZUO Dongjiao1;  GAO Lin1;  CAO Qianwen1;  KONG Fanyun1;  LIU Xiaomei1;  TANG Renxian1;  XUE Min4;  ZHOU Feng1*
1. Jiangsu Key Laboratory of Immunity and Metabolism, Xuzhou, Jiangsu 221004, China; 2.Clinical Laboratory, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009; 3.Department ofBioscience, Xuzhou Medical University, Xuzhou, Jiangsu 221004; 4.Department of Physiology, Xuzhou Medical University, Xuzhou, Jiangsu 221004
关键词:
人类免疫缺陷病毒1型; Tat; 肝细胞; 脂肪代谢; AKT信号通路
Keywords:
human immunodeficiency virus type 1 (HIV-1);  Tat;  hepatocytes;  fat metabolism;  AKT signaling
分类号:
R373.9
文献标志码:
A
摘要:
目的 研究人类免疫缺陷病毒1型（HIV-1）Tat蛋白在调节人肝细胞脂肪代谢基因表达中的作用。方法 根据人肝脏中调节脂质生成的转录因子固醇调节元件结合蛋白-1c（Serbp1c）和碳水化合物调节元件结合蛋白（ChREBP）及其下游基因（Dgat、Lpk、Fasn和Scd-1）的cDNA碱基序列，设计并合成引物；培养人肝细胞株LO2，PI3K/AKT信号通路抑制剂PD98059预处理，Tat蛋白刺激细胞后，提取不同时间点的细胞总RNA，逆转录生成cDNA，应用定量PCR（q-PCR）检测人肝细胞中Srebp1c和ChREBP及其下游基因Dgat、Lpk、Fasn和Scd-1mRNA转录水平。结果 Tat蛋白显著增强人肝细胞中Srebp1c和ChREBP及其下游基因Dgat、Lpk、Fasn和Scd-1 mRNA表达水平，在刺激6h后达到峰值；而且PI3K/AKT信号通路抑制剂PD98059显著下调Tat诱导的Srebp1c和ChREBP及其下游基因Dgat、Lpk、Fasn和Scd-1基因转录。结论 HIV Tat蛋白在人肝细胞中对脂质生成有促进作用，进而调节肝细胞脂肪代谢。
Abstract:
Objective To explore the role of human immunodeficiency virus type 1 (HIV-1) Tat protein in lipid metabolism in human hepatocytes. Methods Primers were designed and synthesized based on the cDNA base sequence of the transcription factor sterol regulatory element binding protein-1c (Serbp1c) and carbohydrate-responsive element binding protein (ChREBP) as well as their target genes (Dgat, Lpk, Fasnand Scd-1), which regulate the regeneration of lipids in human liver. The human hepatocyte cell line LO2 was stimulated with Tat proteins at different time points. Total RNA was extracted and cDNA was generated by reverse transcription. Quantitative PCR (q-PCR) was used to detect the transcription levels of Srebp1c and ChREBP and their downstream genes Dgat, Lpk, Fasn and Scd-1 mRNA in human hepatocytes. Results With the stimulation of HIV-1 Tat protein, the expression levels of Srebp1c and ChREBP and their downstream genes Dgat, Lpk, Fasn and Scd-1 mRNA in human hepatocytes increased significantly, and reached the peak after 6 h. Conclusion HIV-1 Tat protein promotes the regeneration of lipids in human hepatocytes, and regulates the lipid metabolism of hepatocytes.

参考文献/References:

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相似文献/References:

[1]曹春萍、郑哲、左冬姣等.HIV-1Tat激活AKT信号增强肝细胞脂肪代谢基因表达[J].徐州医科大学学报,2018,38(11):701.
　[J].Journal of Xuzhou Medical University,2018,38(11):701.

备注/Memo

备注/Memo:
基金项目：江苏省科技厅自然科学基金面上项目（BK20141136）；国家自然科学基金青年基金（81500914） *通信作者，E-mail：zhoufeng-xzmc@163.com
更新日期/Last Update: 2018-12-06