[1]高原.不同结扎时间对大鼠下肢缺血再灌注损伤模型的影响[J].徐州医科大学学报,2019,39(07):502-506.
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不同结扎时间对大鼠下肢缺血再灌注损伤模型的影响()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
39
期数:
2019年07期
页码:
502-506
栏目:
出版日期:
2019-06-25

文章信息/Info

Title:
Effects of different ligation time on rat lower limb ischemia-reperfusion injury model
作者:
高原
文献标志码:
A
摘要:
目的 建立大鼠下肢急性缺血-再灌注损伤模型,探索夹闭大鼠股动脉时间对该损伤模型的影响,选取合适的夹闭时间建立模型。方法 选取SD大鼠40只,随机分为5组,每组8只,分别做空白对照(Control组)、假手术组(Sham组)、缺血再灌注组(I/R组);其中缺血再灌注组又依据夹闭股动脉分为夹闭3小时组(IR3)、夹闭4小时组(IR4)和夹闭5小时组(IR5)。大鼠麻醉后,将大鼠左后肢游离出股动静脉,用微血管夹将股动静脉夹闭,缝合线结扎股动静脉,分别夹闭3、4、5小时后,恢复血流灌注2小时。并用橡皮带在血管夹下方弹性环扎后肢,将侧支循环阻断,分别在 3h、4h和5h后取下血管夹、结扎线及橡皮带,恢复肢体血流2小时形成再灌注;sham组仅暴露股三角,不结扎动静脉及其分支。每组动物在夹闭结束后的0、2、4、8小时分别取血1.5mL,低温1000×g离心后取血清备用,灌注结束后,取大鼠腓肠肌生理盐水洗净后置于液氮保存备用。结束后分别检测血清中CK、LDH水平,检测腓肠肌MDA、MPO水平。结果 经检测,大鼠在下肢缺血再灌注后,对血中相关指标和肌肉组织相关指标均造成了不同程度的损伤。结论 结扎时间越长,大鼠缺血再灌注损伤越严重,并且恢复所需时间会逐渐增加甚至无法恢复,经比较,选取结扎时间为4小时较为合理。
Abstract:
Objective The model of acute ischemic-reperfusion injury in the lower limbs of rats was established. The effect of clamping the femoral artery time on the injury model was explored. The appropriate clipping time was used to establish the model. Methods Forty SD rats were randomly divided into 5 groups, 8 rats in each group, including blank co ntrol group (Control group), sham operation group (Sham group), ischemia-reperfusion group (I/R group); The reperfusion group was divided into a 3-hour group (IR3), a 4-hour group (IR4), and a 5-hour group (IR5) according to the clipped femoral artery. After anesthesia in rats, the left hind limb of the rat was freed from the femoral artery and vein. The femoral artery was clamped with a microvascular clamp, and the femoral artery and vein were ligated by suture. After 3, 4, and 5 hours of clamping, blood perfusion was resumed for 2 hours. The rubber band was used to elastically circumscribe the hind limbs under the blood vessel clamp, and the collateral circulation was blocked. After 3h, 4h and 5h, the blood vessel clamp, the ligature and the rubber band were removed, and the limb blood flow was restored for 2 hours to form reperfusion; the sham group only exposed the femoral triangle and did not ligature the arteries and veins and their branches. Each group of animals took 1.5 ml of blood at 0, 2, 4, and 8 hours after the end of the clipping. After centrifugation at a low temperature of 1000 × g, the serum was taken for use. After the end of the perfusion, the rat gastrocnemius physiological saline was washed and placed in liquid nitrogen and s ave spare. At the end of the test, the levels of CK and LDH in the serum were measured, and the levels of MDA and MPO in the gastrocnemius muscle were detected. Results After testing, the rats in the lower extremity ischemia and reperfusion, the blood related indicators and muscle tissue related indicators have caused varying degrees of damage. Conclusion The longer the ligation time, the more severe the ischemia-reperfusion injury in rats, and the time required for recovery will gradually increase or even recover. After comparison, it is reasonable to choose a ligation time of 4 hours.
更新日期/Last Update: 2019-07-11