[1]孙明闯,张雨涵,韦艳霞,等.健康宠物狗粪便中分离鉴定索氏梭菌一株[J].徐州医科大学学报,2019,39(08):562-566.
 Isolation and identification of a strain of Clostridium sordelli isolated from the feces of a healthy pet dog.[J].Journal of Xuzhou Medical University,2019,39(08):562-566.
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健康宠物狗粪便中分离鉴定索氏梭菌一株()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
39
期数:
2019年08期
页码:
562-566
栏目:
出版日期:
2019-08-25

文章信息/Info

Title:
Isolation and identification of a strain of Clostridium sordelli isolated from the feces of a healthy pet dog.
作者:
孙明闯张雨涵韦艳霞刘佃滨高静
Keywords:
Clostridium sordelliClostridium difficile16S rDNAphylogenetic treeantimicrobial sensitivity pattern
文献标志码:
A
摘要:
目的 一株菌落形态近似艰难梭菌的未知菌株的鉴别、系统进化及药敏分析。方法 利用艰难梭菌选择性分离培养方法从健康宠物狗粪便中分离纯化菌种。利用艰难梭菌抗原胶乳凝集快速检测试剂盒和艰难梭菌磷酸丙糖异构酶(Triose Phosphate Isomerase,Tpi) 编码基因tpi PCR 扩增进行生化和分子鉴别。PCR扩增未知菌株的16S rDNA 基因、测序进行分子鉴定;利用MEGA 7.0等软件构建系统进化树对其进行系统进化分析,利用琼脂糖稀释法对该菌株进行药物敏感性试验。结果 艰难梭菌选择性培养方法获得的菌落形态疑似艰难梭菌的未知菌株经生化以及分子鉴别后并非艰难梭菌。16S rDNA 序列比对和系统进化分析表明:该菌株系与艰难梭菌同属的索氏梭菌(Clostridium sordelli),与艰难梭菌有较近的亲缘关系,对氨苄西林、克林霉素以及莫西沙星敏感。结论 经艰难梭菌选择性分离培养方法在健康宠物狗粪便中获得菌落形态与艰难梭菌相似的未知菌株为索氏梭菌,其耐药谱型与艰难梭菌差异较大。
Abstract:
Objective To identify an unknown strain, whose clone was similar to Clostridium difficile (C. difficile) by sequencing and phylogenetic analysis of 16S rDNA and test its antimicrobial susceptibility. Methods C. difficile selective medium was used to isolate the strains from fecal samples of healthy pet dogs. To identify unknown strains, a C. difficile latex agglutination rapid test kit was used and the detection of triosephosphate isomerase (tpi) gene by PCR was performed. S equencing of 16S rDNA was used to identify the unknown strain sample and phylogenetic tree was constructed to analyze its evolution. Agarose dilution method was used to evaluate the sensitivity pattern of Clostridium sordelli (C. sordelli). Results The unknown strain was isolated by using C. difficile selective medium. Neither tpi gene nor C. difficile antigen was found, which showed that the unknown strain was not C. difficile. Sequencing and analysis of its 16S rDNA showed that it was C. sordelli. Phylogenetic tree showed that the strain had a relative close evolutionary relationship with C. difficile and it was sensitive to ampicillin, clindamycin and moxifloxacin. Conclusions The unknown strain isolated from a healthy pet dog was C. sordelli. Although phylogenetic tree showed that the strain had a relative close evolutionary relationship with C. difficile, its sensitivity pattern was differed from C. difficile

备注/Memo

备注/Memo:
张雨涵,1996年出生,女,本科在读,研究方向为肠道微生态与转化医学
更新日期/Last Update: 2019-09-18