周围神经损伤后LncRNAs的表达谱分析-《徐州医科大学学报》

文章信息/Info

Title:: Transcriptome study of LncRNAs in the repair of peripheral nerve injury

摘要:: 目的通过基因芯片转录组学分析长链非编码RNA（LncRNAs）在周围神经损伤（PNI）与再生过程中的表达变化及作用。进一步深入了解周围神经损伤与再生机制，为周围神经损伤与再生的治疗提供新的途径。方法本研究使用高通量组学技术检测小鼠坐骨神经损伤后0d、3d、7d、14d远端神经所有mRNAs和lncRNAs的表达变化，并通过生物信息学方法筛选出差异lncRNAs以及差异lncRNAs的靶基因，预测出可能参与神经再生的关键lncRNAs。结果坐骨神经损伤以后，共有5354个lncRNAs表达发生改变。进一步的生物信息学分析得到了最有可能参与神经再生的关键lncRNAs及其靶基因：NONMMUG036609-Cd9，NONMMUG014387-Cthrc1，NONMMUG 035232-Ezh2，ENSMUSG00000087366-Jun， SOX2OT-Sox2，NONMMUG 0105 75- Sox4，为进一步深入研究LncRNAs在周围神经损伤再生中的作用提供了线索和理论基础。结论本研究结果表明，长链非编码RNAs在周围神经损伤和再生的过程中表达发生改变。这些长链非编码RNA可能参与了周围神经损伤后的再生和修复过程，为临床上治疗PNI的提供新的途径和潜在靶点。

Abstract:: ob＜x＞jective To study the role of LncRNAs in repairing peripheral nerve injury (PNI) nerve rege neration through bioinformatic methods,and provide potential therapeutic targets for PNI. Methods High-throughput gene chip technology was used to monitor the gene changes of distal sciatic nerve injury in mice 0day、3day、7day、14day after injury, and the differential genes were screened by bioinformatics method. We investigate the functions of these dysregulated lncRNAs in peripheral nerve damage. Importantly, we predicted that several lncRNA-mRNA pairs may participate in biological processes related to peripheral nerve injury. RT-qPCR was performed for the preliminary verification of three lncRNA?mRNA pairs. Results A total of 5354 lncRNAs with significant ex＜x＞pression differences were detected (p < 0.05; FC ≤ 0.5 or ≥ 2). Among them, NONMMUG036609，NONMMUG01438，NONMMUG 035232，ENSMUSG00000087366， SOX2OT ，NONMMUG010575 may play important role in peripheral nerve regeneration. Conclusions The results of this study indicate that LncRNAs may improve the ability of nerve regeneration. Therefore, our stydy provide a new insight in repairing peripheral nerve injury.