j.issn.2096-3882.2022.03.011]
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SULF1对肝癌细胞增殖的抑制作用及相关分子机制研究()

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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

Title:: Effects of SULF1 on the Proliferation of Hepatocellular Carcinoma Cells and Related Molecular Mechanisms

作者:: 方小康1; 刘志毅1; 2; 曹宽1; 2; 田子路2; 温馨1; 王人颢1; 2*; 1. 徐州医科大学，江苏徐州221004；2. 徐州医科大学附属医院肝胆外科，江苏徐州221002

摘要:: 目的探究硫酸酯酶-1（SULF1）调控肝癌细胞增殖的相关机制。方法通过Western blot和免疫组化实验分析SULF1在正常肝组织和肝癌组织中表达的差异性。在HepG2和HuH-7细胞中构建过表达SULF1的稳转细胞系。通过EdU、CCK-8和集落形成实验探究过表达SULF1对肝癌细胞增殖的影响。通过Western blot探究SULF1调控肝癌细胞增殖的相关分子机制。结果与正常肝组织相比，SULF1在肝癌组织中呈低表达。过表达SULF1可以抑制肝癌细胞的增殖。SULF1可以促进p53的表达。结论SULF1在肝癌中作为一种抑癌基因，通过促进p53抑制肝癌细胞的增殖。

Abstract:: ob＜x＞jective: To explore the related mechanisms of sulfatase-1 (SULF1) in regulating the proliferation of liver cancer cells. Methods: Western Blot and immunohistochemical experiments were used to analyze the difference of SULF1 ex＜x＞pression between normal liver tissue and primary liver cancer tissue. A stable transgenic cell line overexpressing SULF1 was constructed in HepG2 and HuH-7 cells. Edu, CCK-8, and colony formation experiments were performed to investigate the effects of SULF1 overex＜x＞pression on the proliferation of liver cancer cells. Western Blot experiments were conducted to explore the molecular mechanism of SULF1 regulating hepatoma cell proliferation. Results: SULF1 is lowly expressed in liver cancer tissues; overex＜x＞pression of SULF1 inhibits the proliferation of liver cancer cells; SULF1 can promote the ex＜x＞pression of p53. Conclusion: SULF1 acts as a tumor suppressor gene in liver cancer and inhibits the proliferation of liver cancer cells by positively regulating p53.

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更新日期/Last Update: 2022-05-09