Abstract: In the prevention and control of epidemic situations of major infectious diseases, cities generally treat the public health centers as centralized treatment centers and integrate the city’s medical resources for centralized assistance. However, for a city without a public health center, it is possible to quickly requisition a single districts of a multi-hospital districts model hospital and convert it into an admission point for severely ill patients in epidemic prevention and control. This article uses the prefecture-level cities without public health centers in Jiangsu Province as an example to analyze the advantages and disadvantages of the COVID-19’s prevention and control at the designated multi-hospital districts model designated hospitals, and explore the multi-hospital districts model in major infections. The practical effect of the disease epidemic situation, to explore how comprehensive medical institutions in cities without public health centers can cope with the epidemic situation of major infectious diseases, while still maintaining a urban medical treatment capacity.