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    施会敏, 张俊, 李善文, 张爱青, 甘卫华. 原发性肾病综合征患儿肾组织中长链非编码RNA的差异表达研究[J]. 徐州医科大学学报, 2021, 41(2): 79-85. DOI: 10.3969/j.issn.2096-3882.2021.02.001
    引用本文: 施会敏, 张俊, 李善文, 张爱青, 甘卫华. 原发性肾病综合征患儿肾组织中长链非编码RNA的差异表达研究[J]. 徐州医科大学学报, 2021, 41(2): 79-85. DOI: 10.3969/j.issn.2096-3882.2021.02.001
    shu
    Analysis of the differential expression of long noncoding RNAs in children with MCNS[J]. Journal of Xuzhou Medical University, 2021, 41(2): 79-85. DOI: 10.3969/j.issn.2096-3882.2021.02.001
    Citation: Analysis of the differential expression of long noncoding RNAs in children with MCNS[J]. Journal of Xuzhou Medical University, 2021, 41(2): 79-85. DOI: 10.3969/j.issn.2096-3882.2021.02.001
    shu

    原发性肾病综合征患儿肾组织中长链非编码RNA的差异表达研究

    Analysis of the differential expression of long noncoding RNAs in children with MCNS

      摘要
    • 摘要: 目的 原发性肾病综合征是儿童常见的肾小球疾病，微小病变是最常见的病理类型，目前其发病机制尚不完全明确，本研究通过检测长链非编码RNA（lncRNA）在微小病变型肾病综合征（MCNS）患儿肾组织中的改变，探讨lncRNA在微小病变型肾病综合征中的作用。 方法 收集临床微小病变型肾病综合征（MCNS）患儿肾组织标本及对照组儿童肾组织标本各3例，分为正常组（NC组）和疾病组（MCNS组）；应用芯片技术筛选出两组间差异表达的lncRNA，应用RT?PCR 法验证差异表达的lncRNA，并通过生物信息学进一步分析差异表达的lncRNA。 结果 芯片结果显示：与NC组比较，MCNS组有1446条lncRNA高表达，1519条lncRNA低表达（差异倍数>3）；RT-PCR结果显示：与NC组比较，MCNS组ENST00000425497，ENST00000445520，ENST00000451596和uc001goh.1表达显著上调， ENST00000420452，ENST00000445172，ENST00000502435，ENST00000533039，ENST00000546134和NR_033946表达显著下调，与芯片数据一致；生物信息学分析结果显示：差异表达的lncRNA主要涉及钙离子信号通路、MAPK信号通路、核糖体信号通路、氧化磷酸化信号通路等过程。 结论 与对照组比较，MCNS患儿肾组织中的lncRNA表达谱发生了显著变化，提示差异表达的lncRNA可能参与MCNS的发病过程；这一结果可能推进我们目前对相关疾病的认识，为临床诊治及预后判断提供新的思路。

       

      Abstract: objective To investigate the differential expression of long noncoding RNAs (lncRNA) in children with MCNS. Methods Renal tissues were collected from children with MCNS and renal tissue injury, and were divided into two groups: the MCNS group (n=3) and NC group (n=3). The lncRNAs expression levels in two groups were detected by microarray separately, then the differentially expressed lncRNAs were identified by RT?PCR. Further, lncRNAs may be closely related to MCNS development were predicted by analyzing these differentially expressed lncRNAs through a series of bioinformatics. Results Compared with NC group, 1446 lncRNAs showed higher expression and 1519 lncRNAs showed lower ex＜x＞pression in MCNS group(fold change >3.0). RT-PCR results appeared that ENST00000425497，ENST00000445520，ENST00000451596 and uc001goh.1 were significantly up-regulated in MCNS group, and ENST00000420452，ENST00000445172，ENST00000502435，ENST00000533039，ENST00000546134 and NR_033946 were down-regulated. Bioinformatics analysis showed that the differentially expressed lncRNAs mainly involved in calcium signaling pathway, MAPK signaling pathway, Ribosomal pathway, Oxidative phosphorlation, and so on. Conclusion The differential ex＜x＞pression of long noncoding RNAs presents in children with MCNS, suggesting that the differentially expressed lncRNAs may be involved in the pathological process of MCNS and may be used as novel biomarkers for diagnosis and prognosis of MCNS.

       

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