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    汤安群, 江涛, 白津. KIF4A对结直肠癌细胞迁移侵袭的影响及机制研究[J]. 徐州医科大学学报, 2020, 40(10): 710-714. DOI: 10.3969/j.issn.2096-3882.2020.10.002
    引用本文: 汤安群, 江涛, 白津. KIF4A对结直肠癌细胞迁移侵袭的影响及机制研究[J]. 徐州医科大学学报, 2020, 40(10): 710-714. DOI: 10.3969/j.issn.2096-3882.2020.10.002
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    Effect of KIF4A on the migration and invasion of colorectal cancer cells and its mechanism[J]. Journal of Xuzhou Medical University, 2020, 40(10): 710-714. DOI: 10.3969/j.issn.2096-3882.2020.10.002
    Citation: Effect of KIF4A on the migration and invasion of colorectal cancer cells and its mechanism[J]. Journal of Xuzhou Medical University, 2020, 40(10): 710-714. DOI: 10.3969/j.issn.2096-3882.2020.10.002
    shu

    KIF4A对结直肠癌细胞迁移侵袭的影响及机制研究

    Effect of KIF4A on the migration and invasion of colorectal cancer cells and its mechanism

      摘要
    • 摘要: 目的 探讨KIF4A基因对结直肠癌细胞迁移和侵袭能力的影响以及相关机制.方法 分别使用KIF4A-CtrlRNA(si-Ctrl)及KIF4A-siRNA(si-KIF4A)瞬时转染结直肠癌细胞株SW480和DLD1,通过Western blot验证干扰效果.Transwell实验分析KIF4A对结直肠癌细胞的迁移及侵袭能力的影响.Western blot检测迁移侵袭相关蛋白基质金属蛋白酶2(MMP2)、MMP9表达水平的变化.结果 与si-Ctrl组相比,si-KIF4A组细胞的KIF4A蛋白表达明显下降,结直肠癌细胞的迁移及侵袭能力显著下降,迁移侵袭相关蛋白MMP2和MMP9蛋白表达水平显著下降,差异有统计学意义(P<0.001).结论 KIF4A通过促进迁移侵袭相关蛋白MMP2和MMP9的表达来提高结直肠癌细胞的迁移侵袭能力.

       

      Abstract: ob＜x＞jective To investigate the effect and relate d mechanism of KIF4A on migration and invasion of colorectal cancer cells. Methods The KIF4A-CtrlRNA ( si-Ctrl) and KIF4A-siRNA ( si-KIF4A) were transiently transfect ed into colorectal cancer cell lines SW480 and DLD1, and the interference effect was verified by Western blot; the effects of KIF4A on the migration and invasion ability of colorectal cancer cells w ere confirmed with Transwell assay; the changes of migration and invasion-related proteins MMP2 and MMP9 were performed by Western blot. Results Comparing the si-KIF4A group with si-Ctrl group, the KIF4A ex＜x＞pression was significantly reduced; cell proliferation and migration and invasion ability were dramatically decreased ; the ex＜x＞pression levels of MMP2 and MMP9 were significantly decreased in cells . Conclusion KIF4A facilitates migration and invasion ability of colorectal cancer cells through increasing the ex＜x＞pression of MMP2 and MMP9

       

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