[1]耿浩,江涛,徐溢新,等.长链非编码RNA LINC00239在人结直肠癌中的表达及其对结直肠癌细胞HCT116增殖、转移的影响[J].徐州医科大学学报,2021,41(01):1-5.[doi:10.3969/j.issn.2096-3882.2021.01.001]
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长链非编码RNA LINC00239在人结直肠癌中的表达及其对结直肠癌细胞HCT116增殖、转移的影响()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
41
期数:
2021年01期
页码:
1-5
栏目:
出版日期:
2021-01-25

文章信息/Info

Title:
Study on the ex<x>pression of Long Non-Coding RNA LINC00239 in Colorectal Cancer and its effect on proliferation and me<x>tastasis of colorectal cancer cell line HCT116
作者:
耿浩江涛徐溢新董晨花宋虎樊瑞智许腾曹猛宋军徐为
徐州医科大学研究生院,江苏 徐州 221004;徐州医科大学消化病研究所,江苏 徐州 221004;徐州医科大学附属医院普外科,江苏 徐州 221002
关键词:
LINC00239 结直肠癌 增殖 转移 预后
DOI:
10.3969/j.issn.2096-3882.2021.01.001
文献标志码:
A
摘要:
目的 探究 长链非编码RNA (long non-coding RNA,lncRNA) LINC00239 在结直肠癌及其癌旁组织、结直肠癌细胞株及正常结直肠上皮细胞株的表达情况,分析LINC00239对结直肠癌细胞HCT116增殖和迁移、侵袭能力的影响。方法 利用GEO及TCGA数据库分析结直肠癌患者的癌和癌旁组织LINC00239的表达情况,应用实时荧光定量PCR(qRT-PCR)法对LINC00239在结直肠癌和癌旁组织中的表达量进行实验验证。利用GEPIA数据库分析LINC00239表达对结直肠癌患者术后无病生存率的影响。CCK8实验和Transwell迁移、侵袭实验分别检测敲低 LINC00239对结直肠癌细胞增殖和转移能力的影响。 结果 与 癌旁组织比较, LINC00239 在结直肠癌组织的表达明显上调(P<0.001)。LINC00239的表达高低与患者术后无病生存率相关(P=0.033)。与正常结直肠上皮细胞相比,LINC00239在3种结直肠癌细胞株的表达水平明显上调(P<0.05),其中HCT116细胞上调最高(P<0.001)。敲低LINC00239可抑制HCT116细胞的增殖(P<0.001)、迁移(P<0.001)和侵袭(P<0.001)能力。结论 LINC00239在结直肠癌组织和细胞中的表达明显上调,敲低LINC00239可明显抑制结直肠癌HCT116细胞的增殖和转移能力。
Abstract:
ob<x>jective To investigate the relative ex<x>pression of long non-coding RNA (lncRNA) LINC00239 in colorectal cancer and their corresponding normal-appearing tissues, colorectal cancer cell lines and normal colorectal epithelial cell lines, and to investigate its effect on proliferation ability and invasion capability of colorectal cancer HCT116 cell. Methods GEO and TCGA databa<x>se was used to explore the ex<x>pression of LINC00239 in CRC.The relative ex<x>pression of LINC00239 in colorectal cancer tissues and their corresponding normal-appearing tissues was assessed by qRT-PCR. GEPIA databa<x>se was used to analyze the relationship between linc00239 ex<x>pression and disease-free survival rate of colorectal cancer patients. Cell counting kit-8 assay and Transwell assay were used to assess the effects of linc00239 knockdown on the proliferation ability and me<x>tastasis capability of colorectal cancer cell. Results Compared with non-cancerous tissues of adjacent colorectal cancer, LINC00239 ex<x>pression was significantly up-regulated in colorectal cancer tissues (P<0.001). The ex<x>pression of linc00239 was related to the disease-free survival rate of colorectal patient(P=0.033). Compared with normal colorectal epithelial cell lines, the ex<x>pression of LINC00239 in three colorectal cancer cell lines was significantly up-regulated (P<0.05), and the ex<x>pression was the highest in HCT116 cells(P<0.001). Knockdown of LINC00239 ex<x>pression significantly inhibited the proliferation (P<0.001),migration (P<0.001) and invasion capability (P<0.001) of colorectal cancer HCT116 cells. Conclusion Linc00239 was up-regulated in colorectal cancer tissues and colorectal cancer cell lines. Knockdown of linc00239 can suppress the proliferation and me<x>tastasis capability of colorectal cancer HCT116 cells.

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更新日期/Last Update: 2021-03-16