高级检索
    武宸宇, 徐慧, 张蓓. miR-182-5p、TRIM8在子宫内膜癌组织中的表达及其临床意义[J]. 徐州医科大学学报, 2021, 41(12): 893-899. DOI: 10.3969/j.issn.2096-3882.2021.12.006
    引用本文: 武宸宇, 徐慧, 张蓓. miR-182-5p、TRIM8在子宫内膜癌组织中的表达及其临床意义[J]. 徐州医科大学学报, 2021, 41(12): 893-899. DOI: 10.3969/j.issn.2096-3882.2021.12.006
    shu
    ex＜x＞pression and clinical significance of miR-182-5p and TRIM8 in endometrial cancer[J]. Journal of Xuzhou Medical University, 2021, 41(12): 893-899. DOI: 10.3969/j.issn.2096-3882.2021.12.006
    Citation: ex＜x＞pression and clinical significance of miR-182-5p and TRIM8 in endometrial cancer[J]. Journal of Xuzhou Medical University, 2021, 41(12): 893-899. DOI: 10.3969/j.issn.2096-3882.2021.12.006
    shu

    miR-182-5p、TRIM8在子宫内膜癌组织中的表达及其临床意义

    ex＜x＞pression and clinical significance of miR-182-5p and TRIM8 in endometrial cancer

      摘要
    • 摘要: 目的：探讨miRNA-182-5p（miR-182-5p）和三结构域蛋白8（TRIM8）在子宫内膜癌组织中的表达及其临床意义。方法：选取2015年1月-2016年6月于徐州市中心医院行手术治疗的子宫内膜癌的患者76例，收集手术切除的子宫内膜癌组织及相应的癌旁组织，采用免疫组化法检测组织中TRIM8蛋白表达水平，qRT-PCR法检测miR-182-5p、TRIM8 mRNA 表达水平，Spearman法进行相关性分析，Kaplan-Meier法及Cox比例风险回归模型分析子宫内膜癌患者预后影响因素。结果：免疫组化结果显示，子宫内膜癌组织中TRIM8蛋白阳性表达率（55.26%）低于癌旁组织（73.68%）（P＜0.05），qRT-PCR检测结果显示，子宫内膜癌组织中miR-182-5p的表达水平显著高于癌旁组织，而TRIM8的表达水平明显低于癌旁组织（P＜0.05）。子宫内膜癌组织中miR-182-5p、TRIM8表达水平均与FIGO分期、淋巴结转移有关（P＜0.05）。Spearman相关性分析显示，miR-182-5p与TRIM8表达水平呈负相关（r=-0.575，P＜0.05）。Kaplan-Meier分析结果显示，miR-182-5p高表达组、TRIM8低表达组患者的预后较差（P＜0.05）。Cox多因素回归分析显示，miR-182-5p、TRIM8是影响子宫内膜癌患者预后的独立危险因素。结论：子宫内膜癌组织中miR-182-5p高表达，TRIM8低表达，二者的表达失调可能共同参与子宫内膜癌的发生发展，有望成为子宫内膜癌诊疗及预后评估的潜在生物标志物。

       

      Abstract: : ob＜x＞jective: To investigate the ex＜x＞pression and clinical significance of miRNA-182-5p (miR-182-5p) and three-domain protein 8 (TRIM8) in endometrial carcinoma. Methods: A total of 76 patients with endometrial cancer who underwent surgical treatment in Xuzhou Central Hospital from January 2015 to June 2016 were selected. The surgically removed endometrial cancer tissues and corresponding adjacent tissues were collected, and the ex＜x＞pression level of TRIM8 protein in the tissues was detected by immunohistochemical method.The mRNA ex＜x＞pression levels of Mir-182-5p and TRIM8 were detected by QRT-PCR, correlation analysis was performed by Spearman method, and prognostic factors of endometrial cancer were analyzed by Kaplan-Meier method and Cox proportional risk regression model. Results: Immunohistochemical results showed that the positive ex＜x＞pression rate of TRIM8 protein in endometrial cancer tissues (55.26%) was lower than that in adjacent tissues (73.68%) (P < 0.05). QRT-PCR results showed that the ex＜x＞pression level of miR-182-5p in endometrial cancer tissues was significantly higher than that in adjacent tissues.The ex＜x＞pression level of TRIM8 was significantly lower than that of adjacent tissues (P < 0.05).The ex＜x＞pression levels of miR-182-5p and TRIM8 in endometrial cancer tissues were correlated with FIGO stage and lymph node me＜x＞tastasis (P < 0.05).Spearman correlation analysis showed that miR-182-5p was negatively correlated with THE ex＜x＞pression level of TRIM8 (r=-0.575, P < 0.05).Kaplan- meier analysis showed that patients with high Mir-182-5P ex＜x＞pression and low TRIM8 ex＜x＞pression had a poor prognosis (P < 0.05).Cox multivariate regression analysis showed that miR-182-5p and TRIM8 were independent risk factors affecting the prognosis of patients with endometrial cancer. Conclusion: The ex＜x＞pression of miR-182-5p is up-regulated and the ex＜x＞pression of TRIM8 is down-regulated in endometrial cancer tissue. The dysregulation of miR-182-5p and TRIM8 may be involved in the occurrence and development of endometrial cancer, and is expected to become a potential biomarker for the diagnosis, treatment and prognosis assessment of endometrial cancer.

       

      • HTML全文
      • 参考文献(0)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回