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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:

42

期数:

2022年03期

页码:

194-198

栏目:

出版日期:

2022-03-25

文章信息/Info

Title:

ex＜x＞pression and significance of NLRC4 in acute cerebral infarction

作者:

葛纪1; 2; 吴小燕2*; 刘赟2; 张龙2; 李群2; 高金玉2

1. 徐州医科大学附属医院神经内科，江苏徐州221004；2. 中国人民解放军陆军71集团军医院，徐州医科大学附属淮海医院神经内科，江苏徐州221002

关键词:

急性脑梗死; NLRC4; 炎症小体; 白介素-1β; 白介素-18

分类号:

R743.3

DOI:

10.3969/j.issn.2096-3882.2022.03.007

文献标志码:

A

摘要:

目的 探讨 炎症小体NLR家族含CARD结构蛋白4（NLRC4）的表达与急性脑梗死（ACI）之间的关系。 方法 选取 自2019年5月至2020年8月就诊于徐州医科大学附属淮海医院神经内科的39例ACI患者作为研究组，同期进行健康体检的40例患者作为对照组。酶联免疫吸附法（ELISA）检测患者及大鼠外周血中NLRC4、白介素-1β （ IL-1β）及白介素-18（IL-18）的表达水平。构建大鼠ACI动物模型，蛋白免疫印迹试验及组织切片免疫荧光染色评估缺血损伤对大鼠脑组织中NLRC4表达的影响。 结果 两组患者在年龄、体质量指数、性别构成以及吸烟情况方面未见统计学差异（ P＞0.05）；血生化检查项目中，研究组患者的C-反应蛋白较对照组显著升高（P＜0.05），而高密度脂蛋白水平低于对照组（P＜0.05）；研究组患者外周血中NLRC4、IL-1β的表达较对照组显著增高，差异有统计学意义（P＜0.05）；蛋白免疫印迹试验结果显示NLRC4在缺血损伤的大鼠脑组织中表达上调，差异有统计学意义（P＜0.05）；组织切片免疫荧光染色结果显示NLRC4在缺血损伤的神经元细胞中表达增加；ELISA结果显示NLRC4、IL-1β及IL-18在ACI大鼠外周血中的表达水平均高于对照组大鼠（P＜0.05）。 结论 NLRC4及其介导的炎症体信号在ACI脑组织中显著上调，具有作为新型ACI治疗靶点的潜力。

Abstract:

ob＜x＞jective The purpose of this study is to determine the significance of restoration of NLR family CARD domain-containing protein 4 (NLRC4) expressed in acute cerebral infarction (ACI) . Methods 39 cases patients with ACI and 40 healthy controls in our hospital from May 201 9 to August 20 20 were selected as the subjects of the study. The ex＜x＞pression levels of NLRC4, IL-1β and IL-18 in peripheral blood were detected by ELISA. Then, we constructed ACI animal model by blocking the blood flow of middle cerebral artery for 24 hours in rats. Immunofluorescence staining and Western blot were used to detect the ex＜x＞pression of NLRC4 in ischemic and sham operated brain tissue. Results Compared with the control group, the ACI group showed no statistical difference in age, gender, body mass index and smoking (P＞0.05). Blood biochemical tests showed that compared with the control group, C-reactive protein in ACI patients was significantly increased, and high-density lipoprotein was reduced (P＜0.05). ELISA results showed that the levels of NLRC4 and IL-1β in the peripheral blood of ACI patients were significantly up-regulated (P＜0.05). Western blot and immunofluorescence staining confirmed that the ex＜x＞pression of NLRC4 in ischemic-injured neurons increased at the animal level. In addition, the ex＜x＞pression levels of NLRC4, IL-1β and IL-18 in the peripheral blood of ACI rats are higher than those of control rats (P＜0.05). Conclusion NLRC4 is closely related to the occurrence and development of ACI. These discoveries identify the NLRC4 inflammasomes as potential therapeutic targets for stroke

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更新日期/Last Update: 2022-05-09