胃癌患者外周血调节性T细胞比例与免疫状态及预后的关系

高之峰; 李慧; 王庄梅

doi:10.3969/j.issn.2096-3882.2022.04.004

胃癌患者外周血调节性T细胞比例与免疫状态及预后的关系

Relationship between the proportion of regulatory T cells in peripheral blood and the immune status and prognosis in patients with gastric cancer

摘要

摘要: 目的研究外周血调节性T细胞(Treg)比例与胃癌患者临床病理特征、PDL1、HER2表达的相关性及与生存预后的关系。方法回顾性收集徐州市第一人民医院85例胃癌患者和体检中心85例健康对照人群术前或体检时外周血Treg细胞比例数据。分析胃癌患者和健康人群外周血Treg细胞比例以及细胞因子IL-6、IL-17及IL-1β的分泌水平；外周血Treg细胞比例与PDL1、HER2表达的关系；通过生存分析和Cox回归模型研究外周血Treg细胞比例与胃癌患者生存预后的关系。结果健康体检人群Treg细胞比例为（15.3±3.6）%，胃癌患者术前外周血Treg细胞比例为（43.4±9.3）%，胃癌患者Treg比例更高（P<0.01）。健康体检人群外周血IL-6水平为(3.23±1.06) ng/L,IL-17为(1.45±0.36) ng/L,IL-1β为(4.6±1.2) ng/L；胃癌患者术前外周血IL-6水平为(8.41±2.14) ng/L,IL-17为(4.31±1.12) ng/L,IL-1β为(9.3±3.4) ng/L；与健康体检人群比较，胃癌患者术前外周血Treg相关的细胞因子分泌水平更高，差异有统计学意义（P<0.01）。胃癌患者术前外周血Treg细胞高比例与性别（P=0.939）、年龄（P=0.915）、T分期（P=0.144）、PDL1（P=0.588）以及HER2表达（P=0.381）均无关，与N分期以及临床分期有关（P<0.001）。Cox多因素回归模型分析表明，除N分期外（HR=2.299, P=0.015），外周血Treg细胞比例（HR=4.266, P<0.001）可作为判定胃癌患者预后的独立因素。结论通过检测术前外周血Treg细胞比例，可初步判断胃癌患者的预后和机体的免疫状态。

Abstract: ob＜x＞jective: To investigate the correlation between the proportions of peripheral blood Treg cells and the clinicopathological characteristics, PDL1 and HER2 ex＜x＞pression, as well as survival and prognosis in patients with gastric cancer.Methods: Data on the proportion of Treg cells in peripheral blood of 85 healthy controls and 85 patients with gastric cancer in our center were collected retrospectively. The ratio of Treg cells in peripheral blood and the secretion levels of cytokines IL6, IL17 and IL-1 in gastric cancer and normal healthy people were analyzed by t test of two independent samples. Chi-square test was used to analyze the relationship between Treg cell proportion and PDL1 and HER2 ex＜x＞pression in peripheral blood. Survival analysis and COX regression model were used to study the relationship between peripheral blood Treg ratio and survival prognosis of gastric cancer patients.Results: The proportion of Treg cells in healthy physical examination group was 15.3± 3.6%, while 43.4± 9.3% in patients with gastric cancer. The proportion of Treg cells in patients with gastric cancer was higher, and the difference was statistically significant (P<0.001). IL6 was 3.23±1.06 pg/ml, IL17 was 1.45±0.36 pg/ml, and IL-1 was 4.6± 1.2pg /ml in healthy crowd, while 8.41±2.14 pg/ml, 4.31±1.12 pg/ml and 9.3±3.4 pg/ml in patients with gastric cancer before surgery, respectively, showing a statistically significant difference (P<0.001). The high proportion of peripheral blood Treg cells in the preoperative group of gastric cancer was not correlated with gender (P=0.939), age (P=0.915), T stage (P=0.144), PDL1 (P=0.588), and HER2 ex＜x＞pression (P=0.381), but was positively correlated with N stage (P<0.001) and clinical stage (P<0.001), with statistically significant differences. COX regression multivariate model analysis showed that except for N staging (HR=2.299, P=0.015), peripheral blood Treg cell ratio (HR=4.266, P<0.001) could be an independent prognostic factor for gastric cancer patients.Conclusion: By detecting the proportion of Treg cells in peripheral blood before surgery, the immune status of gastric cancer patients can be preliminarily understood, which can be used to judge survival and prognosis.

HTML全文

参考文献(0)

施引文献

资源附件(0)