高级检索
    徐津楠, 朱浩文, 汤爱梦, 孙家立, 叶陆恒, 张咏梅. 下丘脑室旁核CB1R介导急性内脏痛的机制研究[J]. 徐州医科大学学报, 2022, 42(6): 391-395. DOI: 10.3969/j.issn.2096-3882.2022.06.001
    引用本文: 徐津楠, 朱浩文, 汤爱梦, 孙家立, 叶陆恒, 张咏梅. 下丘脑室旁核CB1R介导急性内脏痛的机制研究[J]. 徐州医科大学学报, 2022, 42(6): 391-395. DOI: 10.3969/j.issn.2096-3882.2022.06.001
    Mechanism of acute visceral pain mediated by CB1 receptor[J]. Journal of Xuzhou Medical University, 2022, 42(6): 391-395. DOI: 10.3969/j.issn.2096-3882.2022.06.001
    Citation: Mechanism of acute visceral pain mediated by CB1 receptor[J]. Journal of Xuzhou Medical University, 2022, 42(6): 391-395. DOI: 10.3969/j.issn.2096-3882.2022.06.001

    下丘脑室旁核CB1R介导急性内脏痛的机制研究

    Mechanism of acute visceral pain mediated by CB1 receptor

    • 摘要: 目的探讨下丘脑室旁核(paraventricular nucleus,PVN)大麻素Ⅰ型受体(cannabinoid receptor 1,CB1R)在急性乙酸内脏痛模型中发挥的作用及意义,为临床合理用药提供理论依据。方法选用40只雄性C57小鼠,随机分成5组(n=8),预先通过PVN微量注射给药,建立小鼠急性乙酸内脏痛模型,观察乙酸致小鼠扭体反应次数变化,扭体反应峰值期,采用免疫荧光染色,检测各组小鼠PVN内c-Fos表达水平。结果①乙酸模型组小鼠60 min内的扭体反应次数明显多于正常对照组,说明急性乙酸内脏痛模型建立成功。②与PVN微量注射生理盐水和CB1R拮抗剂AM-251相比,PVN微量注射CB1R激动剂WIN55212-2后,乙酸模型组小鼠在60 min内的扭体反应次数明显减少,并在15~30 min扭体反应达到峰值,小鼠PVN内c-Fos表达明显减少。③与PVN微量注射生理盐水相比,PVN内微量注射CB1R拮抗剂AM-251后,乙酸模型组小鼠在60 min内的扭体反应次数和PVN内c-Fos表达无明显差异,但在15 min内扭体反应达到峰值。结论PVN内预先微量注射CB1R激动剂WIN55212-2可通过抑制PVN内神经元活性减轻小鼠急性乙酸内脏痛。

       

      Abstract: ob<x>jective To investigate the role and significance of cannabinoid receptor 1(CB1R) in hypothalamic paraventricular nucleus (PVN) in acute acetic acid visceral pain model, and to provide theoretical basis for clinical rational drug use. Methods 40 male C57 mice were randomly divided into 5 groups (n = 8). Firstly, the drug was micro injected into PVN, and then the mouse model of acute acetic acid visceral pain was established. The changes of writhing reaction times and peak period of writhing reaction induced by acetic acid were observed. The ex<x>pression level of c-Fos in PVN was detected by immunofluorescence staining. Results ①The number of writhing reactions in acetic acid model group was significantly higher than that in normal control group, indicating that the acute acetic acid visceral pain model was successfully established. ②Compared with PVN micro injection of normal saline, micro injection of CB1R agonist WIN55212-2 into PVN could significantly reduce the number of writhing reaction in acetic acid model group within 60 minutes, and reach the peak time in 15~30 minutes, and significantly reduce the ex<x>pression of c-Fos in PVN. ③After micro injection of CB1R antagonist AM-251 into PVN, there was no significant difference in the number of writhing reaction and the ex<x>pression of c- Fos in PVN in acetic acid model group within 60 minutes, but the writhing reaction reached the peak in 0~15 minutes. Conclusion Microinjection of CB1R agonist WIN55212-2 into PVN can reduce acute acetic acid visceral pain in mice by inhibiting ne uronal activity in PVN

       

    /

    返回文章
    返回