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    丁昕, 娄荷清, 邵晓平, 宋远见, 张训保. HDAC11对脑缺血/再灌注大鼠学习记忆的影响[J]. 徐州医科大学学报, 2017, 37(6): 365-368.
    引用本文: 丁昕, 娄荷清, 邵晓平, 宋远见, 张训保. HDAC11对脑缺血/再灌注大鼠学习记忆的影响[J]. 徐州医科大学学报, 2017, 37(6): 365-368.
    DING Xin, LOU Heqing, SHAO Xiaoping, SONG Yuanjian, ZHANG Xunbao. Effects of HDAC11 on the learning and memory of rats after cerebral ischemia/reperfusion[J]. Journal of Xuzhou Medical University, 2017, 37(6): 365-368.
    Citation: DING Xin, LOU Heqing, SHAO Xiaoping, SONG Yuanjian, ZHANG Xunbao. Effects of HDAC11 on the learning and memory of rats after cerebral ischemia/reperfusion[J]. Journal of Xuzhou Medical University, 2017, 37(6): 365-368.

    HDAC11对脑缺血/再灌注大鼠学习记忆的影响

    Effects of HDAC11 on the learning and memory of rats after cerebral ischemia/reperfusion

    • 摘要: 目的 探讨组蛋白去乙酰化酶11(histone deacetylase 11,HDAC11)对脑缺血/再灌注(I/R)大鼠学习记忆能力的影响。方法 成年雄性SD大鼠随机分为假手术(Sham)组、模型(I/R)组、治疗(MGCD)组、聚乙二醇溶媒对照组(PEG组)。用四血管阻断法制作大鼠全脑I/R模型,Sham组大鼠仅给予双侧椎动脉阻断而不进行颈动脉结扎,MGCD组大鼠于造模前给予侧脑室注射MGCD0103(50 μg/kg)持续1周,PEG组用和给药组相同的给药方法给予PEG处理。于再灌注后的连续5天进行水迷宫实验来检测各组大鼠学习记忆能力的差异。结果 再灌注第2天至第5天,I/R组大鼠的潜伏期均长于Sham组(P<0.05);而MGCD组和I/R组相比,4天的逃避潜伏期均明显缩短(P<0.05)。经过前4天的训练之后,撤去平台,大鼠在第4象限游泳的距离占所有象限的比例:I/R组低于Sham组(P<0.05),MGCD组则高于I/R组(P<0.05)。结论 运用HDAC11抑制剂可以减轻脑I/R对大鼠空间学习记忆能力的

       

      Abstract: Objective To investigate the effects of histone deacetylase 11 (HDAC11) on the learning and memory of rats after cerebral ischemia/reperfusion (I/R). Methods Adult male SD rats were randomly divided into four groups: a sham group, an modeling (I/R) group, a treatment (MGCD) group and a solvent control (PEG) group. A cerebral I/R model of rats was established by the four-vessel occlusion method. Rats in the sham group were occluded at the bilateral arteries without ligation of the carotid. The MGCD group was intracerebroventricularly injected with 50 μg/kg of MGCD0103 for 1 week before modeling. The PEG group was treated with PEG in the same way with the MBDC group. Morris water maze was performed for 5 days after reperfusion to observe the difference in learning and memory among the groups. Results From Days 2 to 5 after reperfusion, the I/R group showed longer latency than the Sham group (P<0.05). Meanwhile, compared with the I/R group, the MGCD group presented remarkably reduced escape latency (P<0.05). On Day 5 after reperfusion, rats were released into the pool without a platform. The percentages of the distance rats swam in the fourth quadrants divided by the distance rats swam in all quadrants were calculated, where the I/R group produced a lower percentage than the Sham group (P<0.05), but the MGCD group produced a higher percentage than the I/R group (P<0.05). Conclusions HDAC11 Inhibitor can relieve the damage of learning and memory in rats after cerebral I/R. HDAC11 may mediate the damage of learning and memory induced by cerebral I/R.

       

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