[1]王金春,张海莲,刘军权,等.Hp感染胃黏膜IL-17、TLR2及PD-1表达及意义[J].徐州医科大学学报,2017,37(10):636-641.
 WANG Jinchun,ZHANG Hailian,LIU Junquan,et al.Expression and significance of IL-17, TLR2 and PD-1 in the gastricmucosa of patients with Hp infection[J].Journal of Xuzhou Medical University,2017,37(10):636-641.
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Hp感染胃黏膜IL-17、TLR2及PD-1表达及意义()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
37
期数:
2017年10期
页码:
636-641
栏目:
出版日期:
2017-10-25

文章信息/Info

Title:
Expression and significance of IL-17, TLR2 and PD-1 in the gastric mucosa of patients with Hp infection
作者:
王金春1张海莲2刘军权2朱云3*
1.徐州医科大学,江苏徐州221004; 2.蚌埠医学院,安徽蚌埠233030;3.中国人民解放军第97医院 生物治疗中心,江苏徐州221004; 4.中国人民解放军第97医院消化科,江苏徐州221004
Author(s):
WANG Jinchun1 ZHANG Hailian2 LIU Junquan2 ZHU Yun3*
1.Xuzhou Medical University, Xuzhou, Jiangsu 221004, China; 2.Bengbu Medical College, Bengbu, Anhui 233030; 3.Biological Therapy Center, the 97th Hospital of Chinese People′s Liberation Army, Xuzhou, Jiangsu 221004; 4.Department of Gastroenterology, the 97th Hospital of Chinese People′s Liberation Army, Xuzhou, Jiangsu 221004
关键词:
幽门螺杆菌萎缩性胃炎白细胞介素-17程序性死亡受体-1Toll样受体
Keywords:
Helicobacter pylori atrophic gastritis interleukin 17 programmed cell death protein 1 toll-like receptor 2
分类号:
R573.3
文献标志码:
A
摘要:
目的探讨幽门螺杆菌(Hp)阳性萎缩性胃炎中胃黏膜免疫损伤机制,为临床寻找根除Hp新的方法或靶点提供理论依据。方法收集胃镜下钳取40例浅表性胃炎(Hp阴性浅表性胃炎20例、Hp阳性浅表性胃炎20例),40例萎缩性胃炎(Hp阴性萎缩性胃炎20例、Hp阳性萎缩性胃炎20例)胃黏膜标本。采用免疫组织化学方法检测白细胞介素-17(IL-17)、Toll样受体2(TLR2)及程序性死亡受体1(PD-1)表达。结果①Hp阳性浅表性胃炎胃黏膜中IL-17、PD-1表达量高于Hp阴性浅表性胃炎,Hp阳性萎缩性胃炎中IL-17、PD-1表达量高于Hp阴性萎缩性胃炎,差异有统计学意义(P<0.05)。②Hp阳性萎缩性胃炎胃黏膜中IL-17、PD-1表达量高于Hp阳性浅表性胃炎,差异有统计学意义(P<0.05)。③各组胃黏膜中TLR2均无表达。结论 Hp感染可上调IL-17、PD-1的表达,Hp感染后疾病的进展与IL-17、PD-1高表达有关,说明IL-17、PD-1在抗Hp的同时具有造成免疫损伤作用。TLR2在Hp感染胃黏膜反应中不参与免疫反应或不参与机体对Hp感染引起免疫应答。
Abstract:
ObjectiveTo investigate the immunological mechanism of mucosa injury in patients with Helicobacter pylori (Hp) positive atrophic gastritis and provided theoretical evidence for new method or targets to eradicate Hp. MethodsMucosa specimen were collected from 40 superficial gastritis patients (20 Hp positive patients and 20 Hp negative patients), and 40 atrophic gastritis patients (20 Hp positive patients and 20 Hp negative patients). The expression of interleukin 17 (IL-17), toll-like receptor 2 (TLR2) and programmed cell death protein 1 (PD-1) was assessed immunohistochemistry. ResultsPatients with Hp positive superficial gastritis showed remarkably higher levels of IL-17 and PD-1 than those with Hp negative superficial gastritis (P<0.05). Patients with Hp positive atrophic gastritis showed remarkably higher levels of IL-17 and PD-1 than those with Hp negative atrophic gastritis (P<0.05). Patients with Hp positive atrophic gastritis showed remarkably higher levels of IL-17 and PD-1 than those with Hp positive superficial gastritis (P<0.05). No statistical difference was found as to TLR2 level in among the groups. ConclusionHp infection can up-regulate the levels of IL-17 and PD-1. The progression of patients with Hp infection is related to high expression of IL-17 and PD-1, which indicated that IL-17 and PD-1 may also cause immunological damage. TLR2 is not involved in immunological responses of Hp infections in the gastric mucosa.

参考文献/References:

1]Roesler BM, Costa SC, Zeitune JM. Eradication treatment of Helicobacter pylori infection: its importance and possible relationship in preventing the development of gastric cancer [J/OL]. SRN Gastroenterol, 2012,2012:935410. doi: 10.5402/2012/935410. [2]中华医学会消化病学分会.中国慢性胃炎共识意见[J].胃肠病学,2013, 18(1):24-36. [3]董晓媛,杨丽霞.慢性萎缩性胃炎幽门螺旋杆菌感染与病理诊断对比分析[J].农垦医学,2015,37(1):41-42. [4]Yan Y, Zhang J, Li JH, et al. High tumor-associated macrophages infiltration is associated with poor prognosis and may contribute to the phenomenon of epithelial-mesenchymal transition in gastric cancer [J/OL]. Onco Targets Ther, 2016,9:3975-3983. doi: 10.2147/OTTS103112. [5]Zhao YR, Zhou Y, Lin G, et al. Association between IL-17, IL-8 and IL-18 expression in peripheral blood and Helicobacter pylori infection in mongolian gerbils [J/OL]. Jundishapur J Microbiol, 2015,8(8):e21503. doi: 10.5812/jjm.21503. [6]Ghosh N, Ghosh P, Kesh K, et al. Attenuation of Helicobacter pylori-induced gastric inflammation by prior cag- strain (AM1) infection in C57BL/6 mice [J]. Gut Pathog, 2017,9:14. [7]Zhang L, Qiu M, Jin Y, et al.Programmed cell death ligand 1 (PD-L1) expression on gastric cancer and its relationship with clinicopathologic factors [J]. Int J Clin Exp Pathol, 2015,8(9):11084-11091. [8]McNab FW, Berry MP, Graham CM, et al. Programmed death ligand 1 is over-expressed by neutrophils in the blood of patients with active tuberculosis [J]. Eur J Immunol, 2011,41(7):1941-1947. [9]Zhou S, Jin X, Li YL, et al. Blockade of PD-1 signaling enhances Th2 cell responses and aggravates liver immunopathology in mice with schistosomiasis japonica [J/OL]. Plos Negl Trop Dis, 2016,10(10):e0005094. doi: 10.1371/journal.pntd.0005094. [10]Khakzad MR, Saffari A,Mohamadpour N,et al.TLR4 and TLR2 expression in biopsy specimens from antral and corporal stomach zones in Helicobacter pylori infections [J]. Rep Biochem Mol Biol, 2014,3(1):29-37. [11]Gong Y, Tao L, Jing L, et al. Association of TLR4 and Treg in Helicobacter pylori colonization and inflammation in mice [J/OL]. PLoS One, 2016,11(2):e0149629. doi: 10.1371/journal.pone.0149629. [12]刁云辉,张建立,冯百岁.幽门螺杆菌感染患者胃黏膜组织中TLR2、TLR5的表达[J].郑州大学学报,2009,44(5):1037-1039. [13]Kalisperati P, Spanou E, Pateras IS,et al. Inflammation, DNA Damage, Helicobacter pylori and Gastric Tumorigenesis [J/OL]. Front Genet, 2017,8:20. doi: 10.3389/fgene.2017.00020. eCollection 2017.

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备注/Memo

备注/Memo:
基金项目:南京军区医学科技创新课题(011Z009) *通信作者,E-mail:zhuyun65@126.com
更新日期/Last Update: 2017-11-16