Abstract: Objective To investigate the effects of aspirin, glycine and lidocaine on acute visceral pain in mice, so as to provide theoretical evidence for rational clinical use. Methods A total of 36 Kunming mice were randomly divided into 6 groups (n=6): a normal saline group (Group NS), an acetic acid model group (Group AA), a morphine + AA group (Group AA+Morphine), an aspirin + AA group (Group AA+Aspirin), a glycine + AA group (Group AA+Gly) and a lidocaine+ AA group (Group AA+Lidocaine). Then, a mouse model of acute visceral pain was established by intraperitoneal injection of 0.6% acetic acid. The latency and counts of acetic acid-induced writhing response were recorded. Results Intraperitoneal injection of acetic acid significantly induced acute visceral pain in mice. Compared with Group AA, mice in Groups AA+Aspirin and AA+Glycine showed significantly prolonged latency (P<0.05); mice in Groups AA+Aspirin, AA+Glycine and AA+Lidocaine showed remarkably decreased counts of writhing response (P<0.05). Conclusions Aspirin, glycine and lidocaine exert an analgesic effect against acute visceral pain in mice induced by acetic acid.