[1]刘晴晴,刘福民.*,刘小云,等.人早孕蜕膜基质细胞表达的SGK1对育龄女性外周血Treg/Th17平衡的影响[J].徐州医科大学学报,2022,42(05):349-352.[doi:10.3969/j.issn.2096-3882.2022.05.007]
 The effect of SGK1 on Treg/Th17 balance in the peripheral blood of women of reproductive age[J].Journal of Xuzhou Medical University,2022,42(05):349-352.[doi:10.3969/j.issn.2096-3882.2022.05.007]
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人早孕蜕膜基质细胞表达的SGK1对育龄女性外周血Treg/Th17平衡的影响()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
42
期数:
2022年05期
页码:
349-352
栏目:
出版日期:
2022-05-25

文章信息/Info

Title:
The effect of SGK1 on Treg/Th17 balance in the peripheral blood of women of reproductive age
作者:
刘晴晴1刘福民2.3*刘小云3冯霞3林华2王风兰2
1. 徐州医科大学研究生院,江苏徐州221004;2. 徐州医科大学附属医院妇产科,江苏徐州221002;3. 徐州医科大学附属医院中心实验室
关键词:
SGK1淋巴细胞调节性T细胞辅助性T细胞免疫耐受
分类号:
R711.5
DOI:
10.3969/j.issn.2096-3882.2022.05.007
文献标志码:
A
摘要:
目的研究蜕膜基质细胞(DSCs)中表达的SGK1对育龄女性外周血淋巴细胞Treg/Th17平衡的影响。方法分离培养正常人早孕DSCs,免疫细胞化学鉴定DSCs,体外分离培养育龄期女性外周血淋巴细胞(PBLC),并分别与各组DSCs共培养。实验分为5组:对照组、共培养组、SGK1激活剂组、 SGK1抑制剂组、共刺激组。Western blot检测各组SGK1蛋白表达水平;逆转录聚合酶链(RT-PCR)法检测SGK1 mRNA表达水平;流式细胞术检测各组Treg与Th17细胞亚群比例。结果Western blot和RT-PCR结果显示,SGK1激活剂组SGK1蛋白以及mRNA表达明显高于其余各组(P<0.05);与共培养组相比,SGK1抑制剂组和共刺激组SGK1蛋白和 mRNA表达明显降低(P<0.05),且抑制剂组降低更明显(P<0.05)。流式细胞术检测显示,SGK1激活剂组Treg细胞亚群/Th17细胞亚群比例明显高于其余4组(P<0.05);与对照组相比,共培养组Treg细胞比例升高且Treg/Th17比例也明显增加;与共培养组相比,SGK1抑制剂组和共刺激组Treg细胞亚群比例明显降低(P<0.05),且SGK1抑制剂组降低更明显(P<0.05)。结论人早孕DSCs表达的SGK1能够影响外周血淋巴细胞中Treg亚群和Th17亚群的比例。
Abstract:
Objective To investigate the effect of SGK1 expression in decidual stromal cells (DSCs) on Treg/Th17 balance of peripheral blood lymphocytes in women of reproductive age. Methods DSCs from normal early pregnancy women were isolated and cultured, and DSCs were identified by immunocytochemistry, then passed to the third or fourth generation. The peripheral blood lymphocytes (PBLC) of women were isolated and cultured in vitro, and co-cultured with each group DSCs. PBLC were divided into 5 groups. 1. Control group, 2. Co-culture group, 3. SGK1 activator group, 4. SGK1 inhibitors group, 5. Co-stimulatory group. The expression levels of SGK1 proteins were detected by Western blotting. The expression levels of SGK1 mRNA were detected by RT-PCR. The proportion of Treg and Th17 cells was measured by flow cytometry. Results Western blotting and RT-PCR results indicate that the expression levels of SGK1 mRNA were significantly higher in SGK-activated group than in the other groups (P < 0.05). Compared with the co-culture group, the levels of SGK1 mRNA both in the SGK1 inhibitor group and the Co-stimulation group were exhibited decreasing trends (P < 0.05), especially lower in the inhibitor group (P<0.05) . Meanwhile, flow cytometry showed that the ration of Treg cells /Th17 cells in the SGK1 activation group was significantly higher than that in the other four groups (P < 0.05). In agreement with the data, the proportion of Treg in in the co-culture group is dramatically increase than control counterpart. Compared with the co-culture group, the percentage of Treg subsets in the SGK1 inhibitor group and the co-stimulus group was obviously reduced (P < 0.05), and the decrease was more significant in the SGK1 inhibitor group. Conclusion SGK1 expressed by DSCs in early pregnancy can affect the proportion of Treg subgroup and Th17 subgroup in peripheral blood lymphocytes。

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备注/Memo

备注/Memo:
基金项目:徐州市科技局社会发展项目(KC16SL108)*通信作者,E-mail: liufumin820@163.com
更新日期/Last Update: 2022-06-07