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    李泳澄, 王翔, 张有为, 袁媛, 韩正祥. PinX1基因对结直肠癌预后及5-FU化疗敏感性的影响[J]. 徐州医科大学学报, 2020, 40(1): 60-64.
    引用本文: 李泳澄, 王翔, 张有为, 袁媛, 韩正祥. PinX1基因对结直肠癌预后及5-FU化疗敏感性的影响[J]. 徐州医科大学学报, 2020, 40(1): 60-64.
    shu
    Effects of PinXlgene on the prognosisand5-FU sensitivity of colorectal cancer[J]. Journal of Xuzhou Medical University, 2020, 40(1): 60-64.
    Citation: Effects of PinXlgene on the prognosisand5-FU sensitivity of colorectal cancer[J]. Journal of Xuzhou Medical University, 2020, 40(1): 60-64.
    shu

    PinX1基因对结直肠癌预后及5-FU化疗敏感性的影响

    Effects of PinXlgene on the prognosisand5-FU sensitivity of colorectal cancer

      摘要
    • 摘要: 目的探讨PinX1(Pin2/TRF1 interacting protein X1)基因表达对结直肠癌(colorectal cancer, CRC)预后及5-氟尿嘧啶（5-fluorouracil, 5-FU）药物敏感性的影响。
      方法GEPIA2在线工具分析PinX1在CRC组织的表达及预后。构建pEGFP-C3-PinX1过表达质粒，分别转染CRC细胞株LoVo和HT29 细胞，CCK-8法检测细胞增殖、测定5-FU 量效曲线并计算IC50，实时定量PCR检测相对端粒长度（relative telomere length, RTL），流式细胞术检测细胞凋亡。
      结果PinX1高表达与CRC分期早及较好的总生存（overall survival,OS）有关。Western blot证实基因转染成功。PinX1过表达组LoVo和HT29细胞的增殖指数降低，5-FU的IC50值降低，RTL降低，凋亡率增加；联合小剂量5-FU（0.5 mg/L）干预后，RTL降低和凋亡增加更为显著。
      结论CRC患者PinX1基因高表达提示预后较好；PinX1过表达可抑制CRC细胞增殖，提高5-FU化疗敏感性，其机制可能与缩短端粒长度、诱导凋亡有关。

       

      Abstract: Objective To explore the effects and mechanism of PinXl gene on the prognosis and 5-fluorouracil (5-FU) sensitivity of colorectal cancer (CRC) . MethodsThe expression status and prognosis of PinX1 in CRC tissueswere analyzed online by GEPIA2 database.Forced expression plasmid pEGFP-C3-PinX1 was constructed and transfected into CRC cell lines LoVo and HT29. CCK-8 was used to determinec ell proliferation, 5-FU dose-response curve and calculate IC50. Real-time quantitative PCR was performed to detect relative telomere length (RTL). Apoptosis was detected by flow cytometry.ResultsHigh expression ofPinX1 is associated with early CRC staging and better overall survival (OS).Western blot verified that PinX1 transfection was successful. The PinX1 overexpression groupsof LoVo and HT29 cells showed a decreased proliferation, IC50 value of 5-FU, the RTL, and anincreased apoptotic rate, compared to the empty vector groups. The RTL decreased andapoptosis increasedmore significantly when combined with low dose5-FU(0.5 μg/mL)treatment.ConclusionUpregulation of PinX1 mRNA indicates better prognosisof CRC. PinX1 overexpression can inhibit the proliferation of CRC cells and increase the sensitivity of 5-FU. The mechanism may be related to shortening telomere length and inducing apoptosis

       

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