[1]李岩.LncRNA XIST靶向miR-15b-5p调控细胞凋亡及自噬参与帕金森发病机制研究[J].徐州医科大学学报,2020,40(07):476-481.
 LncRNA XIST targeted miR-15b-5p participates in the pathogenesis of Parkinson’’s disease by regulating apoptosis and autophagy[J].Journal of Xuzhou Medical University,2020,40(07):476-481.
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LncRNA XIST靶向miR-15b-5p调控细胞凋亡及自噬参与帕金森发病机制研究()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
40
期数:
2020年07期
页码:
476-481
栏目:
出版日期:
2020-07-30

文章信息/Info

Title:
LncRNA XIST targeted miR-15b-5p participates in the pathogenesis of Parkinson’’s disease by regulating apoptosis and autophagy
作者:
李岩
文献标志码:
A
摘要:
目的 探讨LncRNA XIST对帕金森病(PD)的影响及其潜在分子机制。方法 MPTP诱导建立了体内和体外的帕金森模型。用爬杆和转棒法检测小鼠行为变化。MTT和流式细胞术检测SH-SY5Y细胞活力、凋亡。双荧光素酶报告基因法、qRT-PCR、Western blot、免疫组化等方法探讨XIST在帕金森中的分子机制。结果 MPTP诱导的帕金森小鼠脑组织和多巴胺神经元以及MPP+诱导的SH-SY5Y细胞中,XIST表达上调,miR-15b-5p表达下调。沉默XIST可减少往下爬行时间,延长停留时间。此外,沉默BDNF-AS也可增加TH阳性神经元数。miR-15b-5p是XIST的靶基因。si-XIST通过调控miR-15b-5p抑制MPP+诱导SH-SY5Y细胞凋亡和自噬。结论 si-XIST可通过调控miR-15b-5p抑制MPTP诱导的帕金森的自噬和凋亡。
Abstract:
ob<x>jective This study aimed to explore the effect of lncRNA XIST on Parkinson’’s disease (PD). Methods In our study, PD models in vivo and in vitro were established. Mice behavioral changes were detected by pole and rota-rod test. SH-SY5Y cells viability, apoptosis was detected by MTT assay and flow cytometry, respectively. Dual luciferase reporter gene assay, qRT-PCR, Western blot and immunohistochemistry were performed to investigate the molecular mechanism of XIST in PD. Results The ex<x>pression of XIST was upregulated in MPTP-induced brain tissue and dopamine neurons, and MPP+-induced SH-SY5Y cells, but the ex<x>pression of miR-15b-5p was downregualted. Silencing XIST could decrease the T-turn and T-descend time, and increase the retention time. Moreover, silencing XIST could also increase TH positive neurous. miR-15b-5p was the target gene of XIST. si-XIST inhibited MPP+ induced SH-SY5Y cell apoptosis and autophagy by regulating miR-15b-5p. Conclusion si-BDNF-AS could inhibit autophagy and apoptosis in MPTP-induced PD through regulating miR-15b-5p.
更新日期/Last Update: 2020-07-29