Abstract:
ob<x>jective To investigate the effect and mechanism of pirfenidone on the epidural scar cells from epidural area after laminectomy. Methods Tissue block culture was used to culture human epidural scar cells and establish cell lines, and then different concentrations of pirfenidone were added to the cultured cells, Cell viability was measured using cck-8 kit, cell migration was observed by scratch method, cell apoptosis was detected through in situ terminal transferase labeling (TUNEL) staining and the ex<x>pression of TGF-β1, α-SMA, collagen type I, Smad2, phospho-Smad2, Smad3, phospho-Smad3 was measured with western blot. Results Human epidural scar fibroblasts were successfully isolated and cultured. Pirfenidone decreased the activity of fibroblasts (P <0.05), but did not increase the apoptosis of fibroblasts (P> 0.05). Pirfenidone inhibited cell migration and the ex<x>pression of α-SMA, collagen type I protein, Cell proliferation-related proteins and proteins phosphorylation were also inhibit by pirfenidone. Conclusion Pirfenidone inhibit the activity and migration of fibroblasts, and its mechanism may be achieved by inhibiting TGF-β/Smad pathway and myofibroblast transformation