[1]周秀萍,杨新月,徐静,等.YAP靶向抑制剂Verteporfin联合放疗抑制胶质瘤生长的作用与机制研究[J].徐州医科大学学报,2020,40(09):635-639.
 .cn Effect and mechanism of Verteporfin, a YAP targeted inhibitor, combinating with radiotherapy on glioma growth[J].Journal of Xuzhou Medical University,2020,40(09):635-639.
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YAP靶向抑制剂Verteporfin联合放疗抑制胶质瘤生长的作用与机制研究()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
40
期数:
2020年09期
页码:
635-639
栏目:
出版日期:
2020-09-25

文章信息/Info

Title:
.cn Effect and mechanism of Verteporfin, a YAP targeted inhibitor, combinating with radiotherapy on glioma growth
作者:
周秀萍杨新月徐静白雪陈金灿张宇赵敏
文献标志码:
A
摘要:
目的 研究Verteporfin(VP)联合放疗对脑胶质瘤细胞增殖与迁移行为的影响及机制。方法 1. 采用集落形成实验、CCK8实验检测细胞增殖情况。2.采用免疫印迹实验探究VP联合放疗增强放疗效果的机制。3.运用划痕实验检测细胞迁移情况。结果 1. 放疗或VP处理均抑制细胞增殖,放疗联合VP处理的细胞增殖速度更加受到抑制。2.VP联合放疗之后,细胞周期阻滞,增殖减慢。3.放疗或VP处理抑制细胞迁移,放疗联合VP处理后,细胞的迁移能力更加减弱。结论 1.VP 能够提高脑胶质瘤的放疗效果。2.VP联合放疗通过阻滞细胞周期,从而抑制细胞的增殖。
Abstract:
ob<x>jective To study the effect and mechanism of Verteporfin combined with radiotherapy on the proliferation and migration behavior of gliomas. Methods 1. Colony formation assay and CCK8 assay were used to detect the cell proliferation. 2.Western blot was used to explore the Cyr61, CDK4 and p-Rb levels change after VP combined with radiotherapy. 3. Wound healing assay was used to examine the cell migration ability . Results 1. Both radiotherapy and VP treatment inhibited cell proliferation, while radiotherapy combined with VP treatment inhibited cell proliferation even more. 2.After being treated with VP combined with radiotherapy, cell cycle was blocked and proliferation of cells were slowed down. 3.Radiotherapy or VP treatment inhibited cell migration, while radiotherapy combined with VP treatment weakened cell migration ability any more . Conclusion 1.VP can improve the effect of radiotherapy on gliomas. 2.VP combined with radiotherapy blocks the cell cycle, and then inhibits cell proliferation
更新日期/Last Update: 2020-10-12