Abstract:
ob<x>jective To investigate the effect of KIFC1 gene on the proliferation and me<x>tastasis of colorectal cancer. Methods 1. Western blotting was used to detect the ex<x>pression differences of KIFC1 in colorectal cancer and para-carcinoma tissues. 2.Human colorectal cancer cells were transfected with KIFC1-siRNA, then detected the interference effect of KIFC1 in protein ex<x>pression level by western blotting. 3. CCK-8 was used to detect the effect of KIFC1 gene the proliferation of colorectal cancer cells. 4. Transwell assay was conducted to detect the effect of KIFC1 gene on invasion and migration of colorectal cancer cells. 4. Western blotting was used to detect the ex<x>pression of EMT-related proteins, such as E-cadherin, N-cadherin and Vimentin. Results GEPIA databa<x>se and immunoblotting results showed that KIFC1 was highly expressed in colorectal cancer tissue, and KIFC1-siRNA could significantly reduce the ex<x>pression of KIFC1 protein in colorectal cancer cells; CCK-8 experiments showed that KIFC1-siRNA significantly inhibited the proliferation of colorectal cancer cells; Transwell experiments showed that KIFC1-siRNA significantly reduced the me<x>tastatic ability of colorectal cancer cells; inhibiting the ex<x>pression of KIFC1 could promote the ex<x>pression of E-cadherin protein and inhibit the ex<x>pression of N-cadherin and Vimentin protein (P < 0.05). Conclusion Inhibit the ex<x>pression of KIFC1 could inhibit the the proliferation and me<x>tastasis of colorectal cancer cells