[1]杜磊,高红,江涛,等.KIFC1基因对结直肠癌增殖与转移的影响[J].徐州医科大学学报,2020,40(11):790-794.[doi:10.3969/j.issn.2096-3882.2020.11.003]
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KIFC1基因对结直肠癌增殖与转移的影响()
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《徐州医科大学学报》[ISSN:2096-3882/CN:32-1875/R]

卷:
40
期数:
2020年11期
页码:
790-794
栏目:
出版日期:
2020-11-25

文章信息/Info

Title:
The effect of KIFC1 on the proliferationme<x>tastasis of colorectal cancer
作者:
杜磊 高红 江涛 周京宇 孙旭东 宋军
徐州医科大学附属邳州医院,江苏 徐州 221300;徐州医科大学临床学院,江苏 徐州 221004;徐州医科大学附属医院普通外科,江苏 徐州 221002;徐州医科大学消化病研究所,江苏 徐州 221002;徐州医科大学临床学院,江苏 徐州 221004
关键词:
结直肠癌 KIFC1 增殖 转移
DOI:
10.3969/j.issn.2096-3882.2020.11.003
文献标志码:
A
摘要:
目的 探讨KIFC1基因[]对结直肠癌增殖与转移的影响。方法 1.免疫印迹检测KIFC1在结直肠癌与癌旁组织中的表达差异;2.KIFC1-siRNA转染人结直肠癌细胞,免疫印迹检测KIFC1蛋白表达情况;3.CCK-8实验检测KIFC1基因对结直肠癌细胞增殖影响;4.Transwell实验检测KIFC1基因对结直肠癌细胞转移能力的影响。5.免疫印迹检测EMT相关蛋白E-cadherin、N-cadherin、Vimentin表达变化。结果 GEPIA数据库及免疫印迹结果显示KIFC1在结直肠癌组织高表达;与对照组相比,KIFC1-siRNA可显著降低结直肠癌细胞KIFC1蛋白表达;CCK-8实验显示KIFC1-siRNA显著抑制结直肠癌细胞增殖能力;Transwell实验结果显示KIFC1-siRNA显著降低结直肠癌细胞转移能力;同时抑制KIFC1表达,可促进E-cadherin蛋白表达,抑制N-cadherin及Vimentin蛋白表达(P < 0.05)。结论 抑制KIFC1基因表达可以抑制结直肠癌细胞的增殖与转移能力。
Abstract:
ob<x>jective To investigate the effect of KIFC1 gene on the proliferation and me<x>tastasis of colorectal cancer. Methods 1. Western blotting was used to detect the ex<x>pression differences of KIFC1 in colorectal cancer and para-carcinoma tissues. 2.Human colorectal cancer cells were transfected with KIFC1-siRNA, then detected the interference effect of KIFC1 in protein ex<x>pression level by western blotting. 3. CCK-8 was used to detect the effect of KIFC1 gene the proliferation of colorectal cancer cells. 4. Transwell assay was conducted to detect the effect of KIFC1 gene on invasion and migration of colorectal cancer cells. 4. Western blotting was used to detect the ex<x>pression of EMT-related proteins, such as E-cadherin, N-cadherin and Vimentin. Results GEPIA databa<x>se and immunoblotting results showed that KIFC1 was highly expressed in colorectal cancer tissue, and KIFC1-siRNA could significantly reduce the ex<x>pression of KIFC1 protein in colorectal cancer cells; CCK-8 experiments showed that KIFC1-siRNA significantly inhibited the proliferation of colorectal cancer cells; Transwell experiments showed that KIFC1-siRNA significantly reduced the me<x>tastatic ability of colorectal cancer cells; inhibiting the ex<x>pression of KIFC1 could promote the ex<x>pression of E-cadherin protein and inhibit the ex<x>pression of N-cadherin and Vimentin protein (P < 0.05). Conclusion Inhibit the ex<x>pression of KIFC1 could inhibit the the proliferation and me<x>tastasis of colorectal cancer cells

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更新日期/Last Update: 2020-12-15