Abstract:
objective To investigate the differential expression of long noncoding RNAs (lncRNA) in children with MCNS. Methods Renal tissues were collected from children with MCNS and renal tissue injury, and were divided into two groups: the MCNS group (n=3) and NC group (n=3). The lncRNAs expression levels in two groups were detected by microarray separately, then the differentially expressed lncRNAs were identified by RT?PCR. Further, lncRNAs may be closely related to MCNS development were predicted by analyzing these differentially expressed lncRNAs through a series of bioinformatics. Results Compared with NC group, 1446 lncRNAs showed higher expression and 1519 lncRNAs showed lower ex<x>pression in MCNS group(fold change >3.0). RT-PCR results appeared that ENST00000425497,ENST00000445520,ENST00000451596 and uc001goh.1 were significantly up-regulated in MCNS group, and ENST00000420452,ENST00000445172,ENST00000502435,ENST00000533039,ENST00000546134 and NR_033946 were down-regulated. Bioinformatics analysis showed that the differentially expressed lncRNAs mainly involved in calcium signaling pathway, MAPK signaling pathway, Ribosomal pathway, Oxidative phosphorlation, and so on. Conclusion The differential ex<x>pression of long noncoding RNAs presents in children with MCNS, suggesting that the differentially expressed lncRNAs may be involved in the pathological process of MCNS and may be used as novel biomarkers for diagnosis and prognosis of MCNS.