Abstract: Objective To analyze the expression of periostin and Ki67 in cervical carcinoma, evaluate their relationship with the clinical pathological characteristics, and analyze the risk factors that may influence the prognosis of cervical carcinoma patients. Methods A total of 86 cases of cervical carcinoma and 30 cases of normal cervical tissues were collected. The expression of periostin and Ki67 was detected by immunohistochemistry. The relationship between the positive expression of periostin and Ki67 and the clinical pathological characteristics in cervical carcinoma, as well as the relationship between their expressions in cervical carcinoma were analyzed. Then, the risk factors affecting the overall survival after surgery in cervical carcinoma patients were evaluated. Results In 86 cervical carcinoma tissues, the positive rates of periostin and Ki67 were significantly higher than those in normal cervical tissues (P<0.05). The positive expression of periostin was correlated to clinical stage, tumor differentiation, vascular invasion and lymph node metastasis (P<0.05). Similarly, the positive expression of Ki67 was correlated to clinical stage, tumor differentiation, and lymph node metastasis (P<0.05), and there was a positive correlation between the two indexes in cervical carcinoma (γ=0.319, P=0.003). The overall survival was significantly lower in cervical carcinoma patients with positive periostin expression (41.037 months vs 63.672 months, Log-rank test χ²=15.892, P=0.000). The positive expression of periostin was an independent prognostic factor for overall survival in cervical carcinoma patients (HR=3.916, 95% CI: 1.022-15.006, P=0.046<0.05). Conclusions The abnormal expression of periostin and Ki67 may synergistically stimulate the invasion and metastasis of cervical carcinoma. The positive expression of periostin may indicate a poor prognosis in cervical carcinoma.