Abstract:
ob<x>jective To detect the concentrations of KIM-1 and Netrin-1 in urine before and after drug treatment in chronic hepatitis B and observe correlation between KIM-1, Netrin-1 and drug-induced renal dysfuction in chronic hepatitis B. Methods Ninety-five patients with chronic hepatitis B were selected. Fasting blood and morning urine were collected before and after treatment at 12, 24, 36, and 48 weeks with drugs. The ex<x>pression of KIM-1 and Netrin-1 in the urine before and after treatment with drugs was detected by enzyme-li<x>nked immunosorbent assay (ELISA). The levels of HBV-DNA, ALT, creatinine (Cr) and estimated glomerular filtration rate (eGFR) in the serum were measured by using conventional biochemical reagents. Results After treatment with drugs, HBV-DNA content was significantly reduced in chronic hepatitis B, and ALT was also significantly reduced. However, chronic hepatitis B patients treated with drugs for a long time have caused drug-induced kidney injury. Through ELISA assay, the content of KIM-1 in urine of chronic hepatitis B after drug treatment was higher than that before treatment. Similarly, the content of Netrin-1 in urine of chronic hepatitis B after drug treatment was higher than that before treatment. Both urinary KIM-1 and Netrin-1 were positively correlated with changes in Cr and negatively correlated with changes in eGFR. Conclusion The levels of urinary KIM-1 and Netrin-1 are significantly associated with drug-induced renal dysfunction in chronic hepatitis B